Analysis of serum microRNAs (miR-26a-2*, miR-191, miR-337-3p and miR-378) as potential biomarkers in renal cell carcinoma

被引:88
作者
Hauser, Stefan [1 ]
Wulfken, Lena M. [1 ]
Holdenrieder, Stefan [2 ]
Moritz, Rudolf [3 ]
Ohlmann, Carsten-Henning [4 ]
Jung, Volker [4 ]
Becker, Frank [4 ,5 ]
Herrmann, Edwin [3 ]
Walgenbach-Bruenagel, Gisela [2 ]
von Ruecker, Alexander [6 ]
Mueller, Stefan C. [1 ]
Ellinger, Joerg [1 ]
机构
[1] Univ Klinikum Bonn, Klin & Poliklin Urol & Kinderurol, D-53105 Bonn, Germany
[2] Univ Klinikum Bonn, Inst Klin Chem & Klin Pharmakol, D-53105 Bonn, Germany
[3] Univ Klinikum Munster, Klin & Poliklin Urol, D-48149 Munster, Germany
[4] Univ Klinikum Saarlandes, Klin & Poliklin Urol, D-66421 Homburg, Germany
[5] Urol Gemeinschaftspraxis & Ambulatorium Derouet P, D-66538 Neunkirchen, Germany
[6] Univ Klinikum Bonn, Inst Pathol, D-53105 Bonn, Germany
关键词
microRNA; Renal cell carcinoma; Biomarkers; Diagnosis; miR-378; CIRCULATING MICRORNAS; EXPRESSION;
D O I
10.1016/j.canep.2012.04.001
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Introduction: Emerging evidence suggest that microRNAs could serve as non-invasive biomarker for cancer patients. Our study was designed to analyze circulating serum microRNAs in patients with renal cell carcinoma (RCC). Materials and methods: Serum RNA was isolated from patients with clear cell RCC (ccRCC) and non-malignant disease; an artificial microRNA (cel-miR-39) was spiked-in prior the isolation procedure to control isolation efficiency. The levels of miR-26a-2*, miR-191, miR-337-3p and miR-378 in serum were determined using quantitative real-time PCR; the microRNA levels were normalized to cel-miR-39. Results: First, miR-26a-2*, miR-191, miR-337-3p and miR-378 were quantified in serum of each 25 patients with ccRCC and non-malignant disease. The level of miR-378 was significantly increased in ccRCC patients, and thus chosen for validation. The analysis of miR-378 in the validation cohort with 117 RCC patients and 123 control subjects did not confirm a different level of miR-378. Also, miR-378 was not correlated to pT-stage, lymph node/distant metastasis, vascular invasion and Fuhrman grade. Conclusions: The analysis of circulating serum levels of miR-26a-2*, miR-191, miR-337-3p and miR-378 is unlikely to provide helpful diagnostic/prognostic information in RCC patients. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:391 / 394
页数:4
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