Differential expression profiling of microRNAs and their potential involvement in renal cell carcinoma pathogenesis

被引:170
作者
Chow, Tsz-Fung F. [1 ,2 ]
Youssef, Youssef M. [1 ,2 ]
Lianidou, Evi [3 ]
Romaschin, Alexander D. [1 ,2 ,4 ]
Honey, R. John [5 ]
Stewart, Robert [5 ]
Pace, Kenneth T. [5 ]
Yousef, George M. [1 ,2 ,4 ]
机构
[1] St Michaels Hosp, Li Ka Shing Knowledge Inst, Dept Lab Med, Toronto, ON M5B 1W8, Canada
[2] St Michaels Hosp, Li Ka Shing Knowledge Inst, Keenan Res Ctr, Toronto, ON M5B 1W8, Canada
[3] Univ Athens, Dept Chem, Analyt Chem Lab, Athens 15771, Greece
[4] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada
[5] St Michaels Hosp, Dept Urol, Toronto, ON M5B 1W8, Canada
关键词
miRNA; Kidney cancer; Microarray; RT-PCR; Bioinformatics; microRNA; Tumor markers; Renal cell carcinoma; TUMOR-SUPPRESSOR GENE; CANCER; SIGNATURE; HYPOXIA; TARGETS; IDENTIFICATION; TRANSCRIPTION; PATTERNS; ONCOMIRS; KIDNEY;
D O I
10.1016/j.clinbiochem.2009.07.020
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Objective: We seek to identify the differentially expressed miRNAs in the clear cell subtype (ccRCC) of kidney cancer. Design and methods: We performed a miRNA microarray analysis to compare the miRNA expression levels between ccRCC tissues and their normal Counterpart. The top dysregulated miRNAs were validated by quantitative RT-PCR analysis. Bioinformatics analysis was also performed. Results: A total of 33 dysregulated miRNAs were identified in ccRCC, including 21 upregulated miRNAs and many of these miRNAs have been reported to be dysregulated in other malignancies and have a potential role in cancer pathogenesis. The miRNAs showed a significant correlation with reported chromosomal aberration sites. We also utilized target prediction algorithms to identify gene targets. Preliminary analyses showed these targets can be directly involved in RCC pathogenesis. Conclusion: We identified miRNAs that are dysregulated in ccRCC and bioinformatics analysis suggests that these miRNAs may be involved in cancer pathogenesis and have the potential to be biomarkers. (c) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码:150 / 158
页数:9
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