Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1

被引：10

作者：

Du, Yue-feng ^{[1
]}

Liang, Liang ^{[1
]}

Shi, Ying ^{[2
]}

Long, Qing-zhi ^{[1
]}

Zeng, Jin ^{[1
]}

Wang, Xin-yang ^{[1
]}

He, Da-lin ^{[1
]}

机构：

[1] Xi An Jiao Tong Univ, Sch Med, Affiliated Hosp 1, Dept Urol, Xian 710061, Shanxi, Peoples R China

[2] Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Urol, Union Hosp, Wuhan 430074, Hubei, Peoples R China

来源：

GENETICS AND MOLECULAR BIOLOGY | 2012年 / 35卷 / 01期

关键词：

prostate cancer; DNA methylation; DNMT3; RNA interference; MAMMALIAN DNA METHYLTRANSFERASES; RNA INTERFERENCE; GASTRIC-CANCER; METHYLATION; HYPERMETHYLATION; EXPRESSION; EPIGENETICS; INHIBITOR; GROWTH; 3B;

D O I：

10.1590/S1415-47572012005000021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the unfavorable behavior of prostate-cancer cells, thereby implying its potential significance as a promising therapeutic target, with DNMT3A simply in the role of helper.

引用

页码：164 / 171

页数：8

共 28 条

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