A secreted anti-activator, OspD1, and its chaperone, Spa15, are involved in the control of transcription by the type III secretion apparatus activity in Shigella flexneri

被引：99

作者：

Parsot, C ^{[1
]}

Ageron, E ^{[1
]}

Penno, C ^{[1
]}

Mavris, M ^{[1
]}

Jamoussi, K ^{[1
]}

d'Hauteville, H ^{[1
]}

Sansonetti, P ^{[1
]}

Demers, B ^{[1
]}

机构：

[1] Inst Pasteur, INSERM U389, Unite Pathogene Microbienne Mol, F-75724 Paris, France

来源：

MOLECULAR MICROBIOLOGY | 2005年 / 56卷 / 06期

关键词：

D O I：

10.1111/j.1365-2958.2005.04645.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacteria of Shigella spp. are responsible for shigellosis in humans and use a type III secretion ( TTS) system to enter epithelial cells and trigger apoptosis in macrophages. Transit of translocator and effector proteins through the TTS apparatus is activated upon contact of bacteria with host cells. Transcription of approximate to 15 genes encoding effectors is regulated by the TTS apparatus activity and controlled by MxiE, an AraC family activator, and its coactivator IpgC, the chaperone of IpaB and IpaC translocators. Using a genetic screen, we identified ospD1 as a gene whose product negatively controls expression of genes regulated by secretion activity. OspD1 associates with the chaperone Spa15 and the activator MxiE and acts as an anti-activator until it is secreted. The mechanism regulating transcription in response to secretion activity involves an activator ( MxiE), an anti-activator ( OspD1), a co-anti-activator ( Spa15), a coactivator ( IpgC) and two anti-coactivators ( IpaB and IpaC) whose alternative and mutually exclusive interactions are controlled by the duration of the TTS apparatus activity.

引用

页码：1627 / 1635

页数：9

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