Labeling ribonuclease S with a 3 nm Au nanoparticle by two-step assembly

被引：37

作者：

Aubin, ME

Morales, DG

Hamad-Schifferli, K

机构：

[1] MIT, Dept Mech Engn, Cambridge, MA 02139 USA

[2] MIT, Biol Engn Div, Cambridge, MA 02139 USA

来源：

NANO LETTERS | 2005年 / 5卷 / 03期

关键词：

D O I：

10.1021/nl0479031

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We label ribonuclease S with a 3 nm Au nanoparticle (NP) by utilizing its two-piece structure. One portion, S-peptide, is mutated with a unique NP attachment site. NP-peptide self-assembles with the other portion, S-protein, to form an active enzyme. NP mobility decreases with peptide labeling and S-protein association. Surface plasmon shifts support conjugation. Higher S-peptide coverages on the NP surface reduce nonspecific adsorption, while sterically hindering assembly of RNaseS. Thiols displace nonspecific adsorption, maximizing site-specific labeling.

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收藏

页码：519 / 522

页数：4

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