Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail

被引：55

作者：

Nakamura, K

Fujita, A

Murata, T

Watanabe, G

Mori, C

Fujita, J

Watanabe, N

Ishizaki, T

Yoshida, O

Narumiya, S ^{[1
]}

机构：

[1] Kyoto Univ, Fac Med, Dept Pharmacol, Kyoto 606, Japan

[2] Kyoto Univ, Fac Med, Dept Urol, Kyoto 606, Japan

[3] Kyoto Univ, Fac Med, Dept Anat, Kyoto 606, Japan

[4] Kyoto Univ, Fac Med, Dept Clin Mol Biol, Kyoto 606, Japan

来源：

FEBS LETTERS | 1999年 / 445卷 / 01期

关键词：

Rho; rhophilin; testis; sperm; sperm tail; fibrous sheath;

D O I：

10.1016/S0014-5793(99)00087-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tissue distribution and cellular localization of rhophilin, a 71 kDa Rho-binding protein, were examined in mice. Rhophilin mRNA was highly expressed in adult testis, but was absent in the testis of WIWV mice deficient in germ cells.,An anti-rhophilin antibody detected a band of an expected size in sperm extracts, which was enriched in the tail fraction. Immunofluorescence analysis revealed two lines of striated staining running in parallel in the principal piece of the sperm tail, These results suggest that rhophilin is expressed in germ cells and localized in the fibrous sheath of the sperm tail. (C) 1999 Federation of European Biochemical Societies.

引用

页码：9 / 13

页数：5

共 30 条

[1] Rho guanosine triphosphatase mediates the selective stabilization of microtubules induced by lysophosphatidic acid [J].