The product of maize streak virus ORF V1 is associated with secondary plasmodesmata and is first detected with the onset of viral lesions

We have used a polyclonal antiserum derived from a bacterially expressed viral fusion protein to investigate the expression and subcellular localisation of the maize streak virus V1 product (PV1). Western blot analysis of agroinfected tissue showed that PV1 was detectable from 10 days postinoculation, coinciding with the first appearance of chlorotic viral lesions. The viral protein was only detectable in cell wall fractions of plant protein extracts. PV1 migrated with an apparent size of 14 kDa on SDS-PAGE, larger than the 10.9 kDa predicted from the amino acid sequence and therefore suggestive of posttranslational modification. Immunogold labelling located PV1 to the cell walls within lesion tissue and demonstrated a close association between the viral protein and secondary plasmodesmata. These results are consistent with the V1 product of MSV playing a role in the cell-to-cell movement of the virus in infected plants. (C) 1996 Academic Press, Inc.