Mass-spectrometry based minisequencing method for the rapid detection of drug resistance in Mycobacterium tuberculosis

被引：17

作者：

Ikryannikova, Larisa N.

Afanas'ev, Maxim V.

Akopian, Tat'yana A.

Il'ina, Elena N.

Kuz'min, Aleksey V.

Larionova, Elena E.

Smirnova, Tat'yana G.

Chernousova, Larisa N.

Govorun, Vadim M.

机构：

[1] Minist Publ Hlth Russian Federat, Res Inst Phys Chem Med, Moscow 119992, Russia

[2] Russian Acad Med Sci, Cent TB Res Inst, Moscow, Russia

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 70卷 / 03期

关键词：

drug resistance; MALDI-TOF mass-spectrometry; minisequencing; Mycobacterium tuberculosis;

D O I：

10.1016/j.mimet.2007.05.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A MALDI TOF MS based minisequencing method has been developed and applied for the analysis of rifampin (RIF)- and isoniazid (INH)resistant M. tuberculosis strains. Eight genetic markers of RIF resistance-nucleotide polymorphisms located in RRDR of rpoB gene, and three of INH resistance including codon 315 of katG gene and -8 and -15 positions of the promoter region of fabG1-inhA operon were worked out. Based on the analysis of 100 M tuberculosis strains collected from the Moscow region in 1997-2005 we deduced that 91% of RIF-resistant and 94% of INH-resistant strains can be identified using the technique suggested. The approach is rapid, reliable and allows to reveal the drug resistance of M. tuberculosis strains within 12 h after sample isolation. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：395 / 405

页数：11

共 36 条

[31] Detection of a point mutation associated with high-level isoniazid resistance in Mycobacterium tuberculosis by using real-time PCR technology with 3′-minor groove binder-DNA probes
van Doorn, HR
Claas, ECJ
Templeton, KE
van der Zanden, AGM
Vije, ATK
de Jong, MD
Dankert, J
Kuijper, EJ
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (10) : 4630 - 4635
[32] VERESHCHAGIN VA, 2005, NEISSERIA GONORHOEAE, V39, P806
[33] Mechanisms of drug resistance in mycobacterium tuberculosis
Wade, MM
Zhang, Y
[J]. FRONTIERS IN BIOSCIENCE-LANDMARK, 2004, 9 : 975 - 994
[34] World Health Organization, 1998, LAB SERV TUB CONTR 3
[35] Mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis isolates from China
Yue, J
Shi, W
Xie, JP
Li, Y
Zeng, EL
Wang, HH
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (05) : 2209 - 2212
[36] 2003, DECREE MINISTRY PUBL

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