共 17 条
Expression, purification and crystallization of the ammonium transporter Amt-1 from Archaeoglobus fulgidus
被引:10
作者:

Andrade, SLA
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机构:
Univ Gottingen, Inst Mikrobiol & Genet, Abt Mol Strukt Biol, D-37077 Gottingen, Germany Univ Gottingen, Inst Mikrobiol & Genet, Abt Mol Strukt Biol, D-37077 Gottingen, Germany

Dickmanns, A
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Univ Gottingen, Inst Mikrobiol & Genet, Abt Mol Strukt Biol, D-37077 Gottingen, Germany Univ Gottingen, Inst Mikrobiol & Genet, Abt Mol Strukt Biol, D-37077 Gottingen, Germany

Ficner, R
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Univ Gottingen, Inst Mikrobiol & Genet, Abt Mol Strukt Biol, D-37077 Gottingen, Germany Univ Gottingen, Inst Mikrobiol & Genet, Abt Mol Strukt Biol, D-37077 Gottingen, Germany

Einsle, O
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机构:
Univ Gottingen, Inst Mikrobiol & Genet, Abt Mol Strukt Biol, D-37077 Gottingen, Germany Univ Gottingen, Inst Mikrobiol & Genet, Abt Mol Strukt Biol, D-37077 Gottingen, Germany
机构:
[1] Univ Gottingen, Inst Mikrobiol & Genet, Abt Mol Strukt Biol, D-37077 Gottingen, Germany
来源:
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
|
2005年
/
61卷
关键词:
D O I:
10.1107/S1744309105027004
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
Ammonium transporters (Amts) are a class of membrane-integral transport proteins found in organisms from all kingdoms of life. Their key function is the transport of nitrogen in its reduced bioavailable form, ammonia, across cellular membranes, a crucial step in nitrogen assimilation for biosynthetic purposes. The genome of the hyperthermophilic archaeon Archaeoglobus fulgidus has been annotated with three individual genes for ammonium transporters, amt1-3, the roles of which are as yet unknown. The amt1 gene product has been produced by heterologous overexpression in Escherichia coli and the resulting protein has been purified to electrophoretic homogeneity. Crystals of Amt-1 have been obtained by sitting-drop vapour diffusion and diffraction data have been collected.
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页码:861 / 863
页数:3
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