Expression, purification and crystallization of the ammonium transporter Amt-1 from Archaeoglobus fulgidus

被引：10

作者：

Andrade, SLA ^{[1
]}

Dickmanns, A ^{[1
]}

Ficner, R ^{[1
]}

Einsle, O ^{[1
]}

机构：

[1] Univ Gottingen, Inst Mikrobiol & Genet, Abt Mol Strukt Biol, D-37077 Gottingen, Germany

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2005年 / 61卷

关键词：

D O I：

10.1107/S1744309105027004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ammonium transporters (Amts) are a class of membrane-integral transport proteins found in organisms from all kingdoms of life. Their key function is the transport of nitrogen in its reduced bioavailable form, ammonia, across cellular membranes, a crucial step in nitrogen assimilation for biosynthetic purposes. The genome of the hyperthermophilic archaeon Archaeoglobus fulgidus has been annotated with three individual genes for ammonium transporters, amt1-3, the roles of which are as yet unknown. The amt1 gene product has been produced by heterologous overexpression in Escherichia coli and the resulting protein has been purified to electrophoretic homogeneity. Crystals of Amt-1 have been obtained by sitting-drop vapour diffusion and diffraction data have been collected.

引用

页码：861 / 863

页数：3

共 17 条

[11] The human Rhesus-associated RhAG protein and a kidney homologue promote ammonium transport in yeast [J].

Marini, AM ;

Matassi, G ;

Raynal, V ;

André, B ;

Cartron, JP ;

Chérif-Zahar, B .

NATURE GENETICS, 2000, 26 (03) :341-344

[12] Over-production of proteins in Escherichia coli: Mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels [J].

Miroux, B ;

Walker, JE .

JOURNAL OF MOLECULAR BIOLOGY, 1996, 260 (03) :289-298

[13] Nitrogenase: standing at the crossroads [J].

Rees, DC ;

Howard, JB .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2000, 4 (05) :559-566

[14] Gas channels for NH3:: Proteins from hyperthermophiles complement an Escherichia coli mutant [J].

Soupene, E ;

Chu, T ;

Corbin, RW ;

Hunt, DF ;

Kustu, S .

JOURNAL OF BACTERIOLOGY, 2002, 184 (12) :3396-3400

[15] AMMONIUM UPTAKE BY RICE ROOTS .3. ELECTROPHYSIOLOGY [J].

WANG, MY ;

GLASS, ADM ;

SHAFF, JE ;

KOCHIAN, LV .

PLANT PHYSIOLOGY, 1994, 104 (03) :899-906

[16] AmtB is necessary for NH4+-induced nitrogenase switch-off and ADP-ribosylation in Rhodobacter capsulatus [J].

Yakunin, AF ;

Hallenbeck, PC .

JOURNAL OF BACTERIOLOGY, 2002, 184 (15) :4081-4088

[17] The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli [J].

Zheng, L ;

Kostrewa, D ;

Bernèche, S ;

Winkler, FK ;

Li, XD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (49) :17090-17095

← 1 2 →