Fractionation and purification of the phycobiliproteins from Spirulina platensis

被引：75

作者：

Patil, Ganapathi ^{[1
]}

Chethana, S. ^{[1
]}

Madhusudhan, M. C. ^{[1
]}

Raghavarao, K. S. M. S. ^{[1
]}

机构：

[1] Cent Food Technol Res Inst, Dept Food Engn, Mysore 570020, Karnataka, India

来源：

BIORESOURCE TECHNOLOGY | 2008年 / 99卷 / 15期

关键词：

C-phycocyanin; allophycocyanin; aqueous two phase extraction; fractionation; purification;

D O I：

10.1016/j.biortech.2008.01.028

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

C-Phycocyanin and allophycocyanin of Spirulina platensis are fractionated and purified using a non-chromatographic method namely, aqueous two phase extraction for the first time. Optimized process parameters of aqueous two phase extraction (PEG 4000/potassium phosphate of tie line length 18.64% with a phase volume ratio 1.45) resulted in pure C-phycocyanin and allophycocyanin with a purity of 3.23 and 0.74, respectively, in a single extraction. Multiple extractions (two) improved the purity of C-phycocyanin from 3.23 to 4.02. Integration of aqueous two phase extraction with membrane process not only facilitated the separation of phase forming components from the products and also increased the purity of allophycocyanin from 0.74 to 1.5. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：7393 / 7396

页数：4

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