Characterization of Guaiene Synthases from Stellera chamaejasme L. Flowers and Their Application in De novo Production of (-)-Rotundone in Yeast

被引：16

作者：

An, Tianyue ^{[1
,2
]}

Li, Linsheng ^{[1
,2
]}

Lin, Ying ^{[1
]}

Zeng, Fanqi ^{[2
]}

Lin, Pengcheng ^{[3
]}

Zi, Jiachen ^{[1
,2
]}

机构：

[1] Jinan Univ, Biotechnol Inst Chinese Mat Med, Guangzhou 510632, Peoples R China

[2] Jinan Univ, Coll Pharm, Guangzhou 510632, Peoples R China

[3] Qinghai Nationalities Univ, Coll Pharm, Xining 810007, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2020年 / 68卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Stellera chamaejasme L; transcriptome; guaiene synthase; (-)-rotundone; metabolic engineering; yeast; ROTUNDONE CONCENTRATION; WITHIN-VINEYARD; AROMA COMPOUND; PEPPER; RED; SESQUITERPENES; RECONSTRUCTION; BIOSYNTHESIS; SANTALOLS; VOLATILES;

D O I：

10.1021/acs.jafc.9b08303

中图分类号：

S [农业科学];

学科分类号：

082806 [农业信息与电气工程];

摘要：

Four terpene synthases for the biosynthesis of volatile terpenoids were identified from the transcriptome of Stellera chamaejasme L. flowers, including SchTPS1, SchTPS2, SchTPS3, and SchTPS4. Their functions were characterized by synthetic biology approaches in Escherichia coli and in vitro enzymatic assays. SchTPS1, SchTPS2, and SchTPS3 are guaiene synthases, while SchTPS4 is an (E,E)-geranyl linalool synthase. Next, SchTPS1 and a-guaiene 2-oxidase VvSTO2 were co-expressed in Saccharomyces cerevisiae to reconstruct the biosynthetic pathway of (-)-rotundone, which is a unique aroma compound in fruits, vegetables, and wines. This is the first report for the construction of a (-)-rotundone-producing microbial platform.

引用

页码：3214 / 3219

页数：6

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