A tandem repetitive sequence located in the centromeric region of common wheat (Triticum aestivum) chromosomes

被引:47
作者
Kishii, M
Nagaki, K
Tsujimoto, H
机构
[1] Yokohama City Univ, Kihara Inst Biol Res, Totsuka Ku, Yokohama, Kanagawa 2440813, Japan
[2] Yokohama City Univ, Grad Sch Integrated Sci, Totsuka Ku, Yokohama, Kanagawa 2440813, Japan
关键词
centromere; common wheat; TaiI family; tandem repetitive sequences;
D O I
10.1023/A:1016739719421
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although TaiI-family sequences are present in the subtelomeric region of Leymus racemosus, it became apparent in the present study that such sequences are also present in the centromeric region of common wheat (Triticum aestivum). These sequences hybridized to all chromosomes with various degrees of signal strength. FISH using TaiI and Ty3/gypsy, a conservative sequence in cereal centromeres, revealed a complicated arrangement of both sequences in all wheat chromosomes at once. Unlike the Arabidopsis centromeres characterized by massive tandem arrays of 180-bp family with flanking paracentromeric retrotransposons in all chromosomes, wheat chromosomes showed various arrangement patterns of TaiI and Ty3/gypsy sequences depending on the chromosome; TaiI-family sequences were scattered in many wheat centromeres as isolated colonies instead of forming uninterrupted solid tandem arrays. This pattern may have resulted from retrotransposon insertion within pre-existing TaiI-tandem arrays or a two-step amplification mechanism of the TaiI family where each TaiI colony was amplified to form arrays independently after the insertion of TaiI-family sequences along the entire centromere. Although sequence analysis of centromeric TaiI repeats in wheat and subtelomeric TaiI repeats in L. racemosus showed variable and conservative regions between the two repeats, they did not show a distinctive difference phylogenically. The widespread presence of tandem repetitive sequences in the eucaryotic centromere suggests a significant role for them in centromeric formation.
引用
收藏
页码:417 / 428
页数:12
相关论文
共 44 条
[1]   Chromosome-specific molecular organization of maize (Zea mays L.) centromeric regions [J].
Ananiev, EV ;
Phillips, RL ;
Rines, HW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (22) :13073-13078
[2]   A cereal centromeric sequence [J].
AragonAlcaide, L ;
Miller, T ;
Schwarzacher, T ;
Reader, S ;
Moore, G .
CHROMOSOMA, 1996, 105 (05) :261-268
[3]   Sequence analysis of an 80 kb human neocentromere [J].
Barry, AE ;
Howman, EV ;
Cancilla, MR ;
Saffery, R ;
Choo, KHA .
HUMAN MOLECULAR GENETICS, 1999, 8 (02) :217-227
[4]   NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].
BENNETT, MD ;
SMITH, JB .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1976, 274 (933) :227-274
[5]   Multiple repetitive DNA sequences in the paracentromeric regions of Arabidopsis thaliana L. [J].
Brandes, A ;
Thompson, H ;
Dean, C ;
HeslopHarrison, JS .
CHROMOSOME RESEARCH, 1997, 5 (04) :238-246
[6]   CENTROMERE ACTIVATION [J].
BROWN, W ;
TYLERSMITH, C .
TRENDS IN GENETICS, 1995, 11 (09) :337-339
[7]  
CHOO KHA, 1997, CENTROMERE, P50
[8]   Genetic definition and sequence analysis of Arabidopsis centromeres [J].
Copenhaver, GP ;
Nickel, K ;
Kuromori, T ;
Benito, MI ;
Kaul, S ;
Lin, XY ;
Bevan, M ;
Murphy, G ;
Harris, B ;
Parnell, LD ;
McCombie, WR ;
Martienssen, RA ;
Marra, M ;
Preuss, D .
SCIENCE, 1999, 286 (5449) :2468-2474
[9]   Rice (Oryza sativa) centromeric regions consist of complex DNA [J].
Dong, FG ;
Miller, JT ;
Jackson, SA ;
Wang, GL ;
Ronald, PC ;
Jiang, JM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (14) :8135-8140
[10]   Physical arrangement of retrotransposon-related repeats in centromeric regions of wheat [J].
Fukui, KN ;
Suzuki, G ;
Lagudah, ES ;
Rahman, S ;
Appels, R ;
Yamamoto, M ;
Mukai, Y .
PLANT AND CELL PHYSIOLOGY, 2001, 42 (02) :189-196