Cytolethal distending toxin genes in Campylobacter jejuni and Campylobacter coli isolates:: Detection and analysis by PCR

被引：47

作者：

Eyigor, A

Dawson, KA

Langlois, BE

Pickett, CL

机构：

[1] Univ Kentucky, Albert B Chandler Med Ctr, Dept Microbiol & Immunol, Coll Med, Lexington, KY 40536 USA

[2] Univ Kentucky, Dept Anim Sci, Coll Agr, Lexington, KY 40546 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 05期

关键词：

D O I：

10.1128/JCM.37.5.1646-1650.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Campylobacter jejuni produces a toxin called cytolethal distending toxin (CDT). Knowledge of the prevalence and homogeneity of Campylobacter sp. cdt genes is incomplete. In this work, we identified four PCR primer pairs that collectively amplified cdt genes in all of the C. jejuni and Campylobacter call strains tested. Restriction analyses of the cdt PCR products showed clear differences between the cdt genes of these two species, yet there were few heterogeneities noted between members of the same species. Consequently, it may be possible to speciate C. jejuni and C. call isolates on the basis of restriction patterns within their cdt genes.

引用

收藏

页码：1646 / 1650

页数：5

相关论文

共 32 条

[11] THE SEROTYPE AND BIOTYPE DISTRIBUTION OF CLINICAL ISOLATES OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI OVER A 3-YEAR PERIOD [J].

KARMALI, MA ;

PENNER, JL ;

FLEMING, PC ;

WILLIAMS, A ;

HENNESSY, JN .

JOURNAL OF INFECTIOUS DISEASES, 1983, 147 (02) :243-246

[12] VIRULENCE OF CAMPYLOBACTER SPECIES - A MOLECULAR-GENETIC APPROACH [J].

KETLEY, JM .

JOURNAL OF MEDICAL MICROBIOLOGY, 1995, 42 (05) :312-327

[13] Pathogenesis of enteric infection by Campylobacter [J].

Ketley, JM .

MICROBIOLOGY-UK, 1997, 143 :5-21

[14] A POINT-SOURCE OUTBREAK OF CAMPYLOBACTERIOSIS ASSOCIATED WITH CONSUMPTION OF RAW-MILK [J].

KORLATH, JA ;

OSTERHOLM, MT ;

JUDY, LA ;

FORFANG, JC ;

ROBINSON, RA .

JOURNAL OF INFECTIOUS DISEASES, 1985, 152 (03) :592-596

[15] PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples [J].

Linton, D ;

Lawson, AJ ;

Owen, RJ ;

Stanley, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (10) :2568-2572

[16] SEROTYPING OF CAMPYLOBACTER-JEJUNI BY SLIDE AGGLUTINATION BASED ON HEAT-LABILE ANTIGENIC FACTORS [J].

LIOR, H ;

WOODWARD, DL ;

EDGAR, JA ;

LAROCHE, LJ ;

GILL, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 1982, 15 (05) :761-768

[17]

*NAT ADV COMM MICR, 1995, DAIRY FOOD ENV SANIT, V15, P133

[18] Examination of diarrheagenicity of cytolethal distending toxin: Suckling mouse response to the products of the cdtABC genes of Shigella dysenteriae [J].

Okuda, J ;

Fukumoto, M ;

Takeda, Y ;

Nishibuchi, M .

INFECTION AND IMMUNITY, 1997, 65 (02) :428-433

[19] DISTRIBUTION OF THE CYTOLETHAL DISTENDING TOXIN-A GENE (CDTA) AMONG SPECIES OF SHIGELLA AND VIBRIO, AND CLONING AND SEQUENCING OF THE CDT GENE FROM SHIGELLA-DYSENTERIAE [J].

OKUDA, J ;

HISAO, K ;

YOSHIFUMI, T .

MICROBIAL PATHOGENESIS, 1995, 18 (03) :167-172

[20] A new cytolethal distending toxin (CDT) from Escherichia coli producing CNF2 blocks HeLa cell division in G2/M phase [J].

Peres, SY ;

Marches, O ;

Daigle, F ;

Nougayrede, JP ;

Herault, F ;

Tasca, C ;

DeRycke, J ;

Oswald, E .

MOLECULAR MICROBIOLOGY, 1997, 24 (05) :1095-1107

← 1 2 3 4 →