PNA molecular beacons for rapid detection of PCR amplicons

被引：79

作者：

Ortiz, E ^{[1
]}

Estrada, G ^{[1
]}

Lizardi, PM ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Dept Mol Recognit & Struct Biol, Inst Biotecnol, Cuernavaca 62271, Morelos, Mexico

来源：

MOLECULAR AND CELLULAR PROBES | 1998年 / 12卷 / 04期

关键词：

PNA; nucleic-acid detection; fluorescence; microtitre; structured probes;

D O I：

10.1006/mcpr.1998.0175

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The authors have developed a method for rapid detection of polymerase chain reaction (PCR) amplicons based on surface immobilized PNA-DNA hybrid probes ('molecular beacons') that undergo a fluorescent-linked conformational change in the presence of a complementary DNA target. Amplicons can be detected by simply adding a PCR reaction to a microtitre-well containing the previously immobilized probe, and reading the generated fluorescence. No further transfers or washing steps are involved. The authors demonstrate the specificity of the method for the detection of ribosomal DNA from Entamoeba histolytica. (C) 1998 Academic Press.

引用

页码：219 / 226

页数：8

共 12 条

[1] PNA HYBRIDIZES TO COMPLEMENTARY OLIGONUCLEOTIDES OBEYING THE WATSON-CRICK HYDROGEN-BONDING RULES [J].