A method for separation and determination of cytokinin nucleotides from plant tissues

被引：20

作者：

Takei, K ^{[1
]}

Yamaya, T ^{[1
]}

Sakakibara, H ^{[1
]}

机构：

[1] RIKEN, Inst Phys & Chem Res, Plant Sci Ctr, Yokohama, Kanagawa 2300045, Japan

来源：

JOURNAL OF PLANT RESEARCH | 2003年 / 116卷 / 03期

关键词：

cytokinin nucleotides; cytokinins; isopentenyladenine; isopentenyltransferase; liquid chromatography-mass spectrometry; trans-zeatin;

D O I：

10.1007/s10265-003-0099-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Recent progress in the study of cytokinin metabolism in plants indicates that quantitative analysis of cytokinin nucleotides is essential for elucidation of early steps of the biosynthetic pathway. However, traditional procedures for purification and quantification of cytokinin cannot discriminate the various nucleotides. We describe here a method for separation and determination of cytokinin nucleotides through a series of anion-exchange column chromatography steps followed by liquid chromatography-mass spectrometry. This method enabled us to analyze the amount of each species of cytokinin nucleotide in plant tissues.

引用

页码：265 / 269

页数：5

共 21 条

[11] The Tzs protein from Agrobacterium tumefaciens C58 produces zeatin riboside 5′-phosphate from 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate and AMP [J].

Krall, L ;

Raschke, M ;

Zenk, MH ;

Baron, C .

FEBS LETTERS, 2002, 527 (1-3) :315-318

[12] THE BIOSYNTHESIS AND METABOLISM OF CYTOKININS [J].

LETHAM, DS ;

PALNI, LMS .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1983, 34 :163-197

[13] Cytokinin metabolism and action [J].

Mok, DWS ;

Mok, MC .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 2001, 52 :89-118

[14]

Mok M.C., 1994, CYTOKININS CHEM ACTI, P155

[15] Identification of cytokinin biosynthesis genes in Arabidopsis:: A breakthrough for understanding the metabolic pathway and the regulation in higher plants [J].

Sakakibara, H ;

Takei, K .

JOURNAL OF PLANT GROWTH REGULATION, 2002, 21 (01) :17-23

[16]

Schmulling T, 1997, PHYSIOL PLANTARUM, V100, P505, DOI 10.1034/j.1399-3054.1997.1000312.x

[17]

Shaw G., 1994, CYTOKININS CHEM ACTI, P15

[18] Nitrogen-dependent accumulation of cytokinins in root and the translocation to leaf: Implication of cytokinin species that induces gene expression of maize response regulator [J].

Takei, K ;

Sakakibara, H ;

Taniguchi, M ;

Sugiyama, T .

PLANT AND CELL PHYSIOLOGY, 2001, 42 (01) :85-93

[19] Identification of genes encoding adenylate isopentenyltransferase, a cytokinin biosynthesis enzyme, in Arabidopsis thaliana [J].

Takei, K ;

Sakakibara, H ;

Sugiyama, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (28) :26405-26410

[20]

TAKEI K, 1983, J PLANT RES, V116

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