Simultaneous multianalyte detection with a nanometer-scale pore

被引：164

作者：

Kasianowicz, JJ ^{[1
]}

Henrickson, SE ^{[1
]}

Weetall, HH ^{[1
]}

Robertson, B ^{[1
]}

机构：

[1] NIST, Div Biotechnol, Gaithersburg, MD 20899 USA

来源：

ANALYTICAL CHEMISTRY | 2001年 / 73卷 / 10期

关键词：

D O I：

10.1021/ac000958c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

It was recently shown that naturally occurring, genetically engineered or chemically modified channels can be used to detect analytes in solution. We demonstrate here that the overall range of analytes that can be detected by single nanometer-scale pores is expanded using a potentially simpler system, Instead of attaching recognition elements to a channel, they are covalently linked to polymers that otherwise thread through a nanometer-scale pore. Because the rate of unbound polymer entering the pore is proportional to its concentration in the bulk, the binding of analyte to the polymer alters the latter's ability to thread through the pore, and the signal that results from individual polymer translocation is unique to the polymer type; the method permits multianalyte detection and quantitation. We demonstrate here that two different proteins can be simultaneously detected with this technique.

引用

页码：2268 / 2272

页数：5

共 29 条

[1] Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules [J].