Probing α-helical and β-sheet structures of peptides at solid/liquid interfaces with SFG

被引：117

作者：

Chen, XY ^{[1
]}

Wang, J ^{[1
]}

Sniadecki, JJ ^{[1
]}

Even, MA ^{[1
]}

Chen, Z ^{[1
]}

机构：

[1] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

来源：

LANGMUIR | 2005年 / 21卷 / 07期

关键词：

D O I：

10.1021/la050048w

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We demonstrated that sum frequency generation (SFG) vibrational spectroscopy can distinguish different secondary structures of proteins or peptides adsorbed at solid/liquid interfaces. The SFG spectrum for tachyplesin I at the polystyrene (PS)/solution interface has a fingerprint peak corresponding to the B-1/B-3 mode of the antiparallel beta-sheet. This peak disappeared upon the addition of dithiothreitol, which can disrupt the beta-sheet structure. The SFG spectrum indicative of the MS1594 alpha-helical structure was observed at the PS/MS1594 solution interface. This research validates SFG as a powerful technique for revealing detailed secondary structures of interfacial proteins and peptides.

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收藏

页码：2662 / 2664

页数：3

相关论文

共 25 条

[1]

ALBERS WM, 2001, HDB SURFACES INTERFA, P1

[2] The structure, dynamics and orientation of antimicrobial peptides in membranes by multidimensional solid-state NMR spectroscopy [J].

Bechinger, B .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1999, 1462 (1-2) :157-183

[3] Protein deformation of lipid hybrid bilayer membranes studied by sum frequency generation vibrational spectroscopy [J].

Doyle, AW ;

Fick, J ;

Himmelhaus, M ;

Eck, W ;

Graziani, I ;

Prudovsky, I ;

Grunze, M ;

Maciag, T ;

Neivandt, DJ .

LANGMUIR, 2004, 20 (21) :8961-8965

[4] Electronic and molecular properties of an adsorbed protein monolayer probed by two-color sum-frequency generation spectroscopy [J].

Dreesen, L ;

Humbert, C ;

Sartenaer, Y ;

Caudano, Y ;

Volcke, C ;

Mani, AA ;

Peremans, A ;

Thiry, PA ;

Hanique, S ;

Frère, JM .

LANGMUIR, 2004, 20 (17) :7201-7207

[5] The interaction of proteins with solid surfaces [J].

Gray, JJ .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2004, 14 (01) :110-115

[6] Optical spectroscopic investigations of model β-sheet hairpins in aqueous solution [J].

Hilario, J ;

Kubelka, J ;

Keiderling, TA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (25) :7562-7574

[7]

HORBETT TA, 1995, ACS S SERIES, V602

[8] The Vroman effect: A molecular level description of fibrinogen displacement [J].

Jung, SY ;

Lim, SM ;

Albertorio, F ;

Kim, G ;

Gurau, MC ;

Yang, RD ;

Holden, MA ;

Cremer, PS .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (42) :12782-12786

[9] Molecular packing of lysozyme, fibrinogen, and bovine serum albumin on hydrophilic and hydrophobic surfaces studied by infrared-visible sum frequency generation and fluorescence microscopy [J].

Kim, J ;

Somorjai, GA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (10) :3150-3158

[10] Sum-frequency spectroscopy and imaging of aligned helical polypeptides [J].

Knoesen, A ;

Pakalnis, S ;

Wang, M ;

Wise, WD ;

Lee, N ;

Frank, CW .

IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, 2004, 10 (05) :1154-1163