Isolation of isochlorogenic acid isomers in flower buds of Lonicera japonica by high-speed counter-current chromatography and preparative high performance liquid chromatography

被引：35

作者：

Guo, Wei ^{[1
]}

Wang, Liang ^{[1
]}

Gao, Yan ^{[1
]}

Zhao, Bonian ^{[1
]}

Wang, Daijie ^{[2
]}

Duan, Wenjuan ^{[2
]}

Yu, Zongyuan ^{[1
]}

机构：

[1] Shandong Acad Chinese Med, Key Disciplines Anal Tradit Chinese Med, Shandong Key Lab Chinese Med Qual Stand Res, Jinan 250014, Shandong, Peoples R China

[2] Shandong Acad Sci, Shandong Anal & Test Ctr, Jinan 250014, Shandong, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2015年 / 981卷

关键词：

Isochlorogenic acid A; Isochlorogenic acid C; Flower buds of Lonicera japonica; High-speed counter-current chromatography; Preparative high performance liquid chromatography; PURIFICATION; CONSTITUENTS; EXTRACTION;

D O I：

10.1016/j.jchromb.2014.12.020

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

An efficient method was established by HSCCC combined with prep-HPLC for separating isochlorogenic acid isomers from flower buds of Lonicera japonica. The partially purified sample from the methanol extract of flower buds of L. japonica by silica gel column was separated by HSCCC to result in a fraction containing two isochlorogenic acid isomers. The fraction was further isolated by prep-HPLC to yield isochlorogenic acid A and isochlorogenic acid C with purities of 98% and 96%, and the total recoveries at 80.1% and 79.8%, respectively. The chemical structures of isochlorogenic acid isomers were confirmed by electrospray ionization mass spectrometry (ESI-MS) and H-1 nuclear magnetic resonance (NMR). (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：27 / 32

页数：6

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