Liposome-mediated BDNF cDNA transfer in intact and injured rat brain

被引：18

作者：

Iwamoto, Y ^{[1
]}

Yang, K ^{[1
]}

Clifton, GL ^{[1
]}

Hayes, RL ^{[1
]}

机构：

[1] UNIV TEXAS, HLTH SCI CTR, DEPT NEUROSURG, HOUSTON, TX 77030 USA

来源：

NEUROREPORT | 1996年 / 7卷 / 02期

关键词：

gene transfer; liposome; BDNF; cDNA; traumatic brain injury; in vivo; rat;

D O I：

10.1097/00001756-199601310-00056

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

WE examined the temporal profile of the expression of brain-derived neurotrophic factor (BDNF) cDNA containing a viral promoter following the injection of liposome cDNA complexes into the intact and traumatically injured rat brain. In situ hybridization and PCR confirmed the presence of injected BDNF cDNA for at least 6 days after injection. A similar profile of BDNF cDNA was observed when it was injected following cortical impact injury. mRNA was also localized around the injection areas. These results suggest that liposome-mediated delivery of neurotrophin cDNA may be a practical gene transfer method for treating traumatic brain injury.

引用

页码：609 / 612

页数：4

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