Development of a strain-specific SCAR marker for the detection of Trichoderma atroviride 11, a biological control agent against soilborne fungal plant pathogens

被引：55

作者：

Hermosa, MR ^{[1
]}

Grondona, I ^{[1
]}

Díaz-Mínguez, JM ^{[1
]}

Iturriaga, EA ^{[1
]}

Monte, E ^{[1
]}

机构：

[1] Univ Salamanca, Dept Genet & Microbiol, Lab 208, Salamanca 37007, Spain

来源：

CURRENT GENETICS | 2001年 / 38卷 / 06期

关键词：

Trichoderma; biocontrol agents; molecular markers; SCARs;

D O I：

10.1007/s002940000173

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The genus Trichoderma includes biocontrol agents (BCAs) effective against soilborne plant pathogenic fungi. Several potentially useful strains for biological control are difficult to distinguish from other strains of Trichoderma found in the field. So, there is a need to find ways to monitor these strains when applied to natural pathosystems. We have used random amplified polymorphic DNA (RAPD) markers to estimate genetic variation among: sixteen strains of the species T. asperellum, T. atroviride, T, harzianum, T. inhamatum and T. longibrachiatum previously selected as BCAs, and to obtain fingerprinting patterns. Analysis of these polymorphisms revealed four distinct groups, in agreement with previous studies. Some of the RAPD products generated were used to design specific primers. Diagnostic PCR performed using these primers specifically identify the strain T. atroviride 11, showing that DNA markers may be successfully used for identification purposes. This SCAR (sequence-amplified region) marker can clearly 11 from other closely related characterised distinguish strain Trichoderma strains.

引用

页码：343 / 350

页数：8

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