Role of pulse shape in cell membrane electropermeabilization

被引:112
作者
Kotnik, T
Pucihar, G
Rebersek, M
Miklavcic, D
Mir, LM
机构
[1] Inst Gustave Roussy, Lab Vector & Gene Transfer, UMR 8121, CNRS, F-94805 Villejuif, France
[2] Univ Ljubljana, Fac Elect Engn, SI-1000 Ljubljana, Slovenia
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2003年 / 1614卷 / 02期
关键词
electropermeabilization; electroporation; pulse shape; cell survival; cell membrane permeability; molecular uptake;
D O I
10.1016/S0005-2736(03)00173-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The role of the amplitude, number, and duration of unipolar rectangular electric pulses in cell membrane electropermeabilization in vitro has been the subject of several studies. With respect to unipolar rectangular pulses, an improved efficiency has been reported for several modifications of the pulse shape: separate bipolar pulses, continuous bipolar waveforms, and sine-modulated pulses. In this paper, we present the results of a systematic study of the role of pulse shape in permeabilization, cell death, and molecular uptake. We have first compared the efficiency of 1-ms unipolar pulses with rise- and falltimes ranging from 2 to 100 mus, observing no statistically significant difference. We then compared the efficiency of triangular, sine, and rectangular bipolar pulses, and finally the efficiency of sine-modulated unipolar pulses with different percentages of modulation. We show that the results of these experiments can be explained on the basis of the time during which the pulse amplitude exceeds a certain critical value. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:193 / 200
页数:8
相关论文
共 41 条
[1]  
[Anonymous], 2001, RADIOL ONCOL
[2]  
BIEDLER JL, 1970, CANCER RES, V30, P1174
[3]   Elementary [Ca2+]i signals generated by electroporation functionally mimic those evoked by hormonal stimulation [J].
Bobanovic, F ;
Bootman, MD ;
Berridge, MJ ;
Parkinson, NA ;
Lipp, P .
FASEB JOURNAL, 1999, 13 (02) :365-376
[4]   Centriole disassembly in vivo and its effect on centrosome structure and function in vertebrate cells [J].
Bobinnec, Y ;
Khodjakov, A ;
Mir, LM ;
Rieder, CL ;
Eddé, B ;
Bornens, M .
JOURNAL OF CELL BIOLOGY, 1998, 143 (06) :1575-1589
[5]   CELL PORATION AND CELL-FUSION USING AN OSCILLATING ELECTRIC-FIELD [J].
CHANG, DC .
BIOPHYSICAL JOURNAL, 1989, 56 (04) :641-652
[6]   HIGH-EFFICIENCY GENE TRANSFECTION BY ELECTROPORATION USING A RADIOFREQUENCY ELECTRIC-FIELD [J].
CHANG, DC ;
GAO, PQ ;
MAXWELL, BL .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1092 (02) :153-160
[7]   Exploring new instrumentation parameters for electrochemotherapy - Attacking tumors with bursts of biphasic pulses instead of single pulses [J].
Daskalov, I ;
Mudrov, N ;
Peycheva, E .
IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 1999, 18 (01) :62-66
[8]   Cell membrane electropermeabilization with arbitrary pulse waveforms [J].
Flisar, K ;
Puc, M ;
Kotnik, T ;
Miklavcic, D .
IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 2003, 22 (01) :77-81
[9]   Time courses of mammalian cell electropermeabilization observed by millisecond imaging of membrane property changes during the pulse [J].
Gabriel, B ;
Teissié, J .
BIOPHYSICAL JOURNAL, 1999, 76 (04) :2158-2165
[10]   Control by osmotic pressure of voltage-induced permeabilization and gene transfer in mammalian cells [J].
Golzio, M ;
Mora, MP ;
Raynaud, C ;
Delteil, C ;
Teissié, J ;
Rols, MP .
BIOPHYSICAL JOURNAL, 1998, 74 (06) :3015-3022