Potentiometric detection of DNA hybridization

被引：82

作者：

Numnuam, Apon ^{[2
,3
,4
,5
]}

Chumbimuni-Torres, Karin Y. ^{[2
]}

Xiang, Yun ^{[3
,4
]}

Bash, Ralph ^{[3
,4
]}

Thavarungkul, Panote ^{[5
]}

Kanatharana, Proespichaya ^{[5
]}

Pretsch, Ernoe ^{[1
]}

Wang, Joseph ^{[1
,3
,4
]}

Bakker, Eric ^{[1
,2
,6
]}

机构：

[1] ETH, Inst Biogeochem & Pollutant Dynam, CH-8092 Zurich, Switzerland

[2] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

[3] Arizona State Univ, Fulton Sch Engn, Tempe, AZ 85287 USA

[4] Arizona State Univ, Biodesign Inst, Tempe, AZ 85287 USA

[5] Prince Songkla Univ, Fac Sci, Hat Yai 90112, Songkhla, Thailand

[6] Curtin Univ Technol, Dept Appl Chem, Perth, WA 6845, Australia

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1021/ja0775467

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The use of potentiometric microsensors is demonstrated here for the first time to detect DNA hybridization. Cadmium sulfide nanocrystal labels bound on a secondary oligonucleolide are dissolved and detected with cadmium-selective microelectrodes exhibiting DNA detection limits of ca. 2 fmol in a 200 mu sample.

引用

收藏

页码：410 / +

页数：3

相关论文

共 23 条

[1] Liposomes labeled with biotin and horseradish peroxidase: A probe for the enhanced amplification of antigen-antibody or oligonucleotide-DNA sensing processes by the precipitation of an insoluble product on electrodes [J].

Alfonta, L ;

Singh, AK ;

Willner, I .

ANALYTICAL CHEMISTRY, 2001, 73 (01) :91-102

[2] Rational design of potentiometric trace level ion sensors.: A Ag+-selective electrode with a 100 ppt detection limit [J].

Ceresa, A ;

Radu, A ;

Peper, S ;

Bakker, E ;

Pretsch, E .

ANALYTICAL CHEMISTRY, 2002, 74 (16) :4027-4036

[3] Potentiometric biosensing of proteins with ultrasensitive ion-selective microelectrodes and nanoparticle labels [J].

Chumbimuni-Torres, Karin Y. ;

Dai, Zong ;

Rubinova, Nastassia ;

Xiang, Yun ;

Pretsch, Erno ;

Wang, Joseph ;

Bakker, Eric .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (42) :13676-13677

[4] Femtomolar electrochemical detection of DNA targets using metal sulfide nanoparticles [J].

Hansen, JA ;

Mukhopadhyay, R ;

Hansen, JO ;

Gothelf, KV .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (12) :3860-3861

[5] Gene sensor using ferrocenyl oligonucleotide [J].

Ihara, T ;

Nakayama, M ;

Murata, M ;

Nakano, K ;

Maeda, M .

CHEMICAL COMMUNICATIONS, 1997, (17) :1609-1610

[6] Amplified electrical transduction of DNA hybridization based on polymeric beads loaded with multiple gold nanoparticle tags [J].

Kawde, AN ;

Wang, J .

ELECTROANALYSIS, 2004, 16 (1-2) :101-107

[7] Enzyme-amplified electrochemical detection of DNA using electrocatalysis of ferrocenyl-tethered dendrimer [J].

Kim, E ;

Kim, K ;

Yang, H ;

Kim, YT ;

Kwak, J .

ANALYTICAL CHEMISTRY, 2003, 75 (21) :5665-5672

[8] Potentiometry at trace levels in confined samples: Ion-selective electrodes with subfemtomole detection limits [J].

Malon, Adam ;

Vigassy, Tamas ;

Bakker, Eric ;

Pretsch, Erno .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (25) :8154-8155

[9] SEQUENCE-SELECTIVE BIOSENSOR FOR DNA-BASED ON ELECTROACTIVE HYBRIDIZATION INDICATORS [J].

MILLAN, KM ;

MIKKELSEN, SR .

ANALYTICAL CHEMISTRY, 1993, 65 (17) :2317-2323

[10]

NUMNUAM A, IN PRESS ANAL CHEM