Indigo Naturalis Alleviates Dextran Sulfate Sodium-Induced Colitis in Rats via Altering Gut Microbiota

被引：84

作者：

Sun, Zhongmei ^{[1
,2
]}

Li, Junxiang ^{[2
]}

Dai, Yi ^{[3
]}

Wang, Wenting ^{[4
]}

Shi, Rui ^{[2
]}

Wang, Zhibin ^{[2
]}

Ding, Panghua ^{[1
,2
]}

Lu, Qiongqiong ^{[1
,2
]}

Jiang, Hui ^{[2
]}

Pei, Wenjing ^{[2
]}

Zhao, Xingjie ^{[2
]}

Guo, Yi ^{[5
]}

Liu, Jiali ^{[1
,2
]}

Tan, Xiang ^{[2
]}

Mao, Tangyou ^{[2
]}

机构：

[1] Beijing Univ Chinese Med, Grad Sch, Beijing, Peoples R China

[2] Beijing Univ Chinese Med, Dongfang Hosp, Dept Gastroenterol, Beijing, Peoples R China

[3] Hyogo Univ Hlth Sci, Sch Pharm, Dept Pharm, Kobe, Hyogo, Japan

[4] Beijing Yangfangdian Hosp, Dept Tradit Chinese Med, Beijing, Peoples R China

[5] Beijing Univ Chinese Med, Dongzhimen Hosp, Dept Gastroenterol, Beijing, Peoples R China

来源：

FRONTIERS IN MICROBIOLOGY | 2020年 / 11卷

基金：

中国国家自然科学基金; 北京市自然科学基金;

关键词：

gut microbiota; indigo naturalis; dextran sulfate sodium; ulcerative colitis; butyrate; CHAIN FATTY-ACIDS; ULCERATIVE-COLITIS; INTESTINAL MICROBIOTA; TRANSPLANTATION; INFLAMMATION; PROBIOTICS; CHILDREN; EFFICACY; IMMUNITY;

D O I：

10.3389/fmicb.2020.00731

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Ulcerative colitis is a gastrointestinal disorder intricately associated with intestinal dysbiosis, but effective treatments are currently limited. Indigo naturalis, a traditional Chinese medicine derived from indigo plants, has been widely used in the treatment of ulcerative colitis. However, the specific mechanisms have not yet been identified. Accordingly, in this study, we evaluated the effects and mechanisms of indigo naturalis on dextran sulfate sodium (DSS)-induced colitis in rats. Our results showed that indigo naturalis potently alleviated DSS-induced colitis in rats, and reversed DSS-induced intestinal dysbiosis using bacterial 16S rRNA amplicon sequencing. The protective effects of indigo naturalis were gut microbiota dependent, as demonstrated by antibiotic treatments and fecal microbiota transplantation. Depletion of the gut microbiota through a combination of antibiotic treatments blocked the anti-inflammatory effect of indigo naturalis on the DSS-induced colitis, and the recipients of the gut microbiota from indigo naturalis-treated rats displayed a significantly attenuated intestinal inflammation, which was actively responsive to therapeutic interventions with indigo naturalis. Notably, supplement with indigo naturalis greatly increased the levels of feces butyrate, which was positively correlated with the relative abundances of Ruminococcus_1 and Butyricicoccus. We further showed that indigo naturalis-dependent attenuation of colitis was associated with elevated expression of short-chain fatty acid-associated receptors GPR41 and GPR43. Collectively, these results suggested that indigo naturalis alleviates DSS-induced colitis in rats through a mechanism of the microbiota-butyrate axis, particularly alterations in Ruminococcus_1 and Butyricicoccus abundances, and target-specific microbial species may have unique therapeutic promise for ulcerative colitis.

引用

页数：16

共 66 条

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