An Improved Flp Deleter Mouse in C57Bl/6 Based on Flpo Recombinase

被引：108

作者：

Kranz, Andrea

Fu, Jun

Duerschke, Kristin

Weidlich, Stefanie ^{[1
]}

Naumann, Ronald ^{[2
]}

Stewart, A. Francis ^{[1
]}

Anastassiadis, Konstantinos ^{[1
]}

机构：

[1] Tech Univ Dresden, BioInnovat Zentrum, Ctr Regenerat Therapies Dresden, D-01307 Dresden, Germany

[2] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany

来源：

GENESIS | 2010年 / 48卷 / 08期

关键词：

site specific recombination; conditional mutagenesis; genome engineering; allele design; SITE-SPECIFIC RECOMBINATION; EMBRYONIC STEM-CELLS; MAMMALIAN-CELLS; ESCHERICHIA-COLI; MICE; CRE;

D O I：

10.1002/dvg.20641

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Recently, a codon improved version of the Flpe site specific recombinase, termed Flpo, was reported as having greatly improved performance in mammalian cell applications. However, the degree of improvement could not be estimated because essentially no Flpe activity was observed. Here, we compare Flpe and Flpo accurately in a mammalian cell assay to estimate that Flpo is about five times more active than Flpe and similar to Cre and Dre. Consequently, we generated a Flpo deleter mouse line from the JM8 C57BI/6 ES cells used in the EUCOMM and KOMP systematic knock-out programs. In breeding experiments, we show that the Flpo deleter delivers complete recombination using alleles that are incompletely recombined by a commonly used Flpe deleter. This indicates that the Flpo deleter is more efficient. genesis 48:512-520, 2010. (C) 2010 Wiley-Liss, Inc.

引用

页码：512 / 520

页数：9

共 20 条

[1] Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E-coli, mammalian cells and mice [J].