Interaction behaviors between chitosan and hemoglobin

被引：51

作者：

Chen, Liuhua ^{[1
]}

Liu Tianqing ^{[1
]}

机构：

[1] Yangzhou Univ, Sch Chem & Chem Engn, Yangzhou 25002, Jinagsu, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2008年 / 42卷 / 05期

基金：

中国国家自然科学基金;

关键词：

interaction; chitosan; hemoglobin;

D O I：

10.1016/j.ijbiomac.2008.02.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction and association between chitosan and hemoglobin (Hb) are studied by UV-vis and fluorescence spectroscopies, viscometry, circular dichroism, dynamic and static light scattering. Chitosan can obviously associate with Hb to form protein-chitosan complexes, which affects microstructure of Hb. The distance between the first association site of chitosan with 214-tryptophan residue in Hb is about 5.473 nm. The intrinsic UV-vis absorption and fluorescence intensities of Hb increase with an increase of chitosan concentration. The alpha-helix in Hb is drawn and changed into P-sheet. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：441 / 446

页数：6

共 52 条

[1] Tyrosinase-catalyzed grafting of sericin peptides onto chitosan and production of protein-polysaccharide bioconjugates [J].

Anghileri, Anna ;

Lantto, Raija ;

Kruus, Kristiina ;

Arosio, Cristina ;

Freddi, Giuliano .

JOURNAL OF BIOTECHNOLOGY, 2007, 127 (03) :508-519

[2] Electrochemical studies of rutin interacting with hemoglobin and determination of hemoglobin [J].

Bao, XY ;

Zhu, ZW ;

Li, NQ ;

Chen, JG .

TALANTA, 2001, 54 (04) :591-596

[3]

BERDE CB, 1979, J BIOL CHEM, V254, P391

[4] Characterization of chitosan by steric exclusion chromatography [J].

Brugnerotto, J ;

Desbrières, J ;

Roberts, G ;

Rinaudo, M .

POLYMER, 2001, 42 (25) :9921-9927

[5] Chitosan as a polymer for pH-induced DNA capture in a totally aqueous system [J].

Cao, Weidong ;

Easley, Christopher J. ;

Ferrance, Jerome P. ;

Landers, James P. .

ANALYTICAL CHEMISTRY, 2006, 78 (20) :7222-7228

[6] Rheological properties of chitin/lithium chloride, N,N-dimethyl acetamide solutions [J].

Chen, BQ ;

Sun, K ;

Zhang, KB .

CARBOHYDRATE POLYMERS, 2004, 58 (01) :65-69

[7] Effect of temperature on the intrinsic viscosity and conformation of chitosans in dilute HCl solution [J].

Chen, RH ;

Tsaih, ML .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1998, 23 (02) :135-141

[8] PH-DEPENDENT INTERACTIONS BETWEEN ADSORBED CHITOSAN LAYERS [J].

CLAESSON, PM ;

NINHAM, BW .

LANGMUIR, 1992, 8 (05) :1406-1412

[9] Fluorescent investigation of the interactions between N-(p-chlorophenyl)-N'-(1-naphthyl) thiourea and serum albumin:: Synchronous fluorescence determination of serum albumin [J].

Cui, Feng-Ling ;

Wang, Jun-Li ;

Cui, Yan-Rul ;

Li, J'an-Ping .

ANALYTICA CHIMICA ACTA, 2006, 571 (02) :175-183

[10] Complex coacervation of proteins and anionic polysaccharides [J].

de Kruif, CG ;

Weinbreck, F ;

de Vries, R .

CURRENT OPINION IN COLLOID & INTERFACE SCIENCE, 2004, 9 (05) :340-349

← 1 2 3 4 5 6 →