Dynamic long-range chromatin interactions control Myb proto-oncogene transcription during erythroid development

被引：111

作者：

Stadhouders, Ralph ^{[1
]}

Thongjuea, Supat ^{[2
]}

Andrieu-Soler, Charlotte ^{[1
,3
]}

Palstra, Robert-Jan ^{[1
]}

Bryne, Jan Christian ^{[2
]}

van den Heuvel, Anita ^{[1
]}

Stevens, Mary ^{[1
]}

de Boer, Ernie ^{[1
]}

Kockx, Christel ^{[4
]}

van der Sloot, Antoine ^{[4
]}

van den Hout, Mirjam ^{[4
]}

van IJcken, Wilfred ^{[4
]}

Eick, Dirk ^{[5
]}

Lenhard, Boris ^{[2
,6
]}

Grosveld, Frank ^{[1
,7
]}

Soler, Eric ^{[1
,7
]}

机构：

[1] Erasmus MC, Dept Cell Biol, NL-3015 GE Rotterdam, Zuid Holland, Netherlands

[2] Univ Bergen, Uni Comp AS, Computat Biol Unit, Bergen, Norway

[3] INSERM, U872, Paris, France

[4] Erasmus MC, Ctr Biom, Rotterdam, Netherlands

[5] Ctr Integrated Prot Sci CIPSM, Helmholtz Zentrum Munchen, Dept Mol Epigenet, Munich, Germany

[6] Univ Bergen, Dept Biol, Bergen, Norway

[7] Erasmus MC, Canc Genom Ctr, Rotterdam, Netherlands

来源：

EMBO JOURNAL | 2012年 / 31卷 / 04期

关键词：

ChIP-sequencing (ChIP-Seq); chromosome conformation capture-sequencing (3C-Seq); erythroid development; long-range interactions; Myb; BETA-GLOBIN LOCUS; FETAL-HEMOGLOBIN LEVELS; GENOME-WIDE ANALYSIS; RNA-POLYMERASE-II; FACTOR C-MYB; GENE-EXPRESSION; HEMATOPOIETIC DEVELOPMENT; MESSENGER-RNA; SELF-RENEWAL; FACTOR EKLF;

D O I：

10.1038/emboj.2011.450

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The key haematopoietic regulator Myb is essential for coordinating proliferation and differentiation. ChIP-Sequencing and Chromosome Conformation Capture (3C)-Sequencing were used to characterize the structural and protein-binding dynamics of the Myb locus during erythroid differentiation. In proliferating cells expressing Myb, enhancers within the Myb-Hbs1l intergenic region were shown to form an active chromatin hub (ACH) containing the Myb promoter and first intron. This first intron was found to harbour the transition site from transcription initiation to elongation, which takes place around a conserved CTCF site. Upon erythroid differentiation, Myb expression is downregulated and the ACH destabilized. We propose a model for Myb activation by distal enhancers dynamically bound by KLF1 and the GATA1/TAL1/LDB1 complex, which primarily function as a transcription elongation element through chromatin looping. The EMBO Journal (2012) 31, 986-999. doi: 10.1038/emboj.2011.450; Published online 13 December 2011

引用

页码：986 / 999

页数：14

共 59 条

[1] TIF1γ Controls Erythroid Cell Fate by Regulating Transcription Elongation [J].

Bai, Xiaoying ;

Kim, Jonghwan ;

Yang, Zhongan ;

Jurynec, Michael J. ;

Akie, Thomas E. ;

Lee, Joseph ;

LeBlanc, Jocelyn ;

Sessa, Anna ;

Jiang, Hong ;

DiBiase, Anthony ;

Zhou, Yi ;

Grunwald, David J. ;

Lin, Shuo ;

Cantor, Alan B. ;

Orkin, Stuart H. ;

Zon, Leonard I. .

CELL, 2010, 142 (01) :133-143

[2] Critical functions for c-Myb at three checkpoints during thymocyte development [J].

Bender, TP ;

Kremer, CS ;

Kraus, M ;

Buch, T ;

Rajewsky, K .

NATURE IMMUNOLOGY, 2004, 5 (07) :721-729

[3] DIFFERENTIAL EXPRESSION OF C-MYB MESSENGER-RNA IN MURINE-B LYMPHOMAS BY A BLOCK TO TRANSCRIPTION ELONGATION [J].

BENDER, TP ;

THOMPSON, CB ;

KUEHL, WM .

SCIENCE, 1987, 237 (4821) :1473-1476

[4] Modifications of RNA polymerase II are pivotal in regulating gene expression states [J].

Brookes, Emily ;

Pombo, Ana .

EMBO REPORTS, 2009, 10 (11) :1213-1219

[5] NARWHAL, a primary analysis pipeline for NGS data [J].

Brouwer, R. W. W. ;

van den Hout, M. C. G. N. ;

Grosveld, F. G. ;

van IJcken, W. F. J. .

BIOINFORMATICS, 2012, 28 (02) :284-285

[6] Progression through the RNA Polymerase II CTD Cycle [J].

Buratowski, Stephen .

MOLECULAR CELL, 2009, 36 (04) :541-546

[7] Hematopoietic development: a balancing act [J].

Cantor, AB ;

Orkin, SH .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 2001, 11 (05) :513-519

[8] The active spatial organization of the β-globin locus requires the transcription factor EKLF [J].

Drissen, R ;

Palstra, RJ ;

Gillemans, N ;

Splinter, E ;

Grosveld, F ;

Philipsen, S ;

de Laat, W .

GENES & DEVELOPMENT, 2004, 18 (20) :2485-2490

[9] Progression through key stages of haemopoiesis is dependent on distinct threshold levels of c-Myb [J].

Emambokus, N ;

Vegiopoulos, A ;

Harman, B ;

Jenkinson, E ;

Anderson, G ;

Frampton, J .

EMBO JOURNAL, 2003, 22 (17) :4478-4488

[10] Discovering Hematopoietic Mechanisms through Genome-wide Analysis of GATA Factor Chromatin Occupancy [J].

Fujiwara, Tohnu ;

O'Geen, Henriette ;

Keles, Sunduz ;

Blahnik, Kimberly ;

Linnemann, Amelia K. ;

Kang, Yoon-A. ;

Choi, Kyunghee ;

Farnham, Peggy J. ;

Bresnick, Emery H. .

MOLECULAR CELL, 2009, 36 (04) :667-681

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