Development and characterization of polymorphic microsatellite markers from Fragaria viridis, a wild diploid strawberry

被引：60

作者：

Sargent, DJ ^{[1
]}

Hadonou, AM

Simpson, DW

机构：

[1] Hort Res Int, W Malling ME19 6BJ, Kent, England

[2] Univ Reading, Sch Plant Sci, Reading RG6 6AS, Berks, England

来源：

MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 04期

关键词：

Fragaria; microsatellite; molecular markers; Rosaceae; strawberry;

D O I：

10.1046/j.1471-8286.2003.00507.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To date, the development of microsatellite (SSR) markers in the genus Fragaria has focused on F. vesca. However, further species are thought to have contributed to the complex allo-octoploid genome of the cultivated strawberry, F. x ananassa. Here, we present 22 new SSR markers developed from the diploid species F. viridis. Twenty-one of the primer pairs amplified polymorphisms in six F. viridis accessions, with an average of 4.95 alleles per primer pair and an average expected heterozygosity of 0.68. Fourteen of these primer pairs, and a locus monomorphic in F. viridis, amplified polymorphic alleles in the parents of a F. vesca mapping population.

引用

页码：550 / 552

页数：3

共 6 条

[1]

[Anonymous], 1998, Primer 3

[2] Optimisation of a microsatellite enrichment technique in Saccharum spp. [J].