Isolation and characterization of polymorphic microsatellites in diploid strawberry (Fragaria vesca L.) for mapping, diversity studies and clone identification

被引：43

作者：

James, CM ^{[1
]}

Wilson, F ^{[1
]}

Hadonou, AM ^{[1
]}

Tobutt, KR ^{[1
]}

机构：

[1] Hort Res Int, W Malling ME19 6BJ, Kent, England

来源：

MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 02期

关键词：

Fragaria vesca; microsatellite; Rosaceae; strawberry;

D O I：

10.1046/j.1471-8286.2003.00365.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study reports the development and characterization of 10 polymorphic microsatellite primer pairs in wild strawberry (Fragaria vesca). The primers were designed from a genomic library enriched for di-, tri- and tetranucleotide repeats from F. vesca 'Reugen'. They showed single locus polymorphism in a set of nine F. vesca accessions; two to six alleles were detected per locus. The level of polymorphism in F. vesca was surprisingly low, although three pairs of primers were sufficient to distinguish between most accessions.

引用

页码：171 / 173

页数：3

共 10 条

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[2] Nuclear DNA amounts in angiosperms and their modern uses - 807 new estimates [J].