Long-lasting RNAi activity in mammalian neurons

被引：70

作者：

Omi, K

Tokunaga, K

Hohjoh, H

机构：

[1] Natl Ctr Neurol & Psychiat, Natl Inst Neurosci, Kodaira, Tokyo 1878502, Japan

[2] Univ Tokyo, Grad Sch Med, Dept Human Genet, Bunkyo Ku, Tokyo 1130033, Japan

来源：

FEBS LETTERS | 2004年 / 558卷 / 1-3期

基金：

日本学术振兴会;

关键词：

RNA interference; mammalian neuron; synthetic small interfering RNA; long double-stranded RNA; apoptosis;

D O I：

10.1016/S0014-5793(04)00017-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of RNA interference (RNAi) induced by synthetic small interfering RNAs (siRNAs) on proliferating mammalian cells appears to last for approximately 3-7 days after its induction. Here we show that the RNAi activity induced by a synthetic 21-nucleotide siRNA duplex in postmitotic neurons, mouse primary hippocampal neurons and neurons that differentiated from mouse embryonal carcinoma P19 cells persists for at least 3 weeks, suggesting long-lasting RNAi activity in mammalian neurons. In addition, we also show that an apoptotic (or antiviral) pathway triggered by long dsRNAs is generated during neuronal differentiation of P19 cells, by which the sequence-specific RNAi activity involving long dsRNA appears to be masked. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：89 / 95

页数：7

共 32 条

[11] An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells [J].