Binding of misacylated tRNAs to the ribosomal A site

被引：10

作者：

Dale, T ^{[1
]}

Uhlenbeck, OC ^{[1
]}

机构：

[1] Northwestern Univ, Dept Biochem Mol Biol & Cell Biol, Evanston, IL 60208 USA

来源：

RNA | 2005年 / 11卷 / 11期

关键词：

tRNA; ribosome; uniformity; misacylated tRNA; A site; ribosome binding;

D O I：

10.1261/rna.2130505

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To test whether the ribosome displays specificity for the esterified amino acid and the tRNA body of an aminoacyl-tRNA (aa-tRNA), the stabilities of 4 correctly acylated and 12 misacylated tRNAs in the ribosomal A site were determined. By introducing the GAC (valine) anticodon into each tRNA, a constant anticodon-codon interaction was maintained, thus removing concern that different anticodon-codon strengths might affect the binding of the different aa-tRNAs to the A site. Surprisingly, all 16 aa-tRNAs displayed similar dissociation rate constants from the A site. These results suggest that either the ribosome is not specific for different amino acids and tRNA bodies when intact aa-tRNAs are used or the specificity for the amino acid side chain and tRNA body is masked by a conformational change upon aa-tRNA release.

引用

页码：1610 / 1615

页数：6

共 37 条

[1] Predicting the binding affinities of misacylated tRNAs for Thermus thermophilus EF-Tu•GTP [J].

Asahara, H ;

Uhlenbeck, OC .

BIOCHEMISTRY, 2005, 44 (33) :11254-11261

[2] The tRNA specificity of Thermus thermophilus EF-Tu [J].

Asahara, H ;

Uhlenbeck, OC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (06) :3499-3504

[3] Specificity of RNA-RNA helix recognition [J].

Battle, DJ ;

Doudna, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (18) :11676-11681

[4] AMINOACYL DERIVATIVES OF NUCLEOSIDES, NUCLEOTIDES AND POLYNUCLEOTIDES .33. STEREOCHEMICAL CONTROL OF RIBOSOMAL PEPTIDYLTRANSFERASE REACTION - ROLE OF AMINO-ACID SIDE-CHAIN ORIENTATION OF ACCEPTOR SUBSTRATE [J].

BHUTA, A ;

QUIGGLE, K ;

OTT, T ;

RINGER, D ;

CHLADEK, S .

BIOCHEMISTRY, 1981, 20 (01) :8-15

[5] tRNA selection and kinetic proofreading in translation [J].

Blanchard, SC ;

Gonzalez, RL ;

Kim, HD ;

Chu, S ;

Puglisi, JD .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2004, 11 (10) :1008-1014

[6] An active role for tRNA in decoding beyond codon:anticodon pairing [J].

Cochella, L ;

Green, R .

SCIENCE, 2005, 308 (5725) :1178-1180

[7] RATES OF AMINOACYL-TRANS-RNA SELECTION AT 29 SENSE CODONS INVIVO [J].

CURRAN, JF ;

YARUS, M .

JOURNAL OF MOLECULAR BIOLOGY, 1989, 209 (01) :65-77

[8] The affinity of elongation factor Tu for an aminoacyl-tRNA is modulated by the esterified amino acid [J].

Dale, T ;

Sanderson, LE ;

Uhlenbeck, OC .

BIOCHEMISTRY, 2004, 43 (20) :6159-6166

[9] Enlarging the amino acid set of Escherichia coli by infiltration of the valine coding pathway [J].

Döring, V ;

Mootz, HD ;

Nangle, LA ;

Hendrickson, TL ;

de Crécy-Lagard, V ;

Schimmel, P ;

Marlière, P .

SCIENCE, 2001, 292 (5516) :501-504

[10] Uniform binding of aminoacylated transfer RNAs to the ribosomal A and P sites [J].

Fahlman, RP ;

Dale, T ;

Uhlenbeck, OC .

MOLECULAR CELL, 2004, 16 (05) :799-805

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