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ELISPOT for detecting antibody-secreting cells in response to infections and vaccination
被引:15
作者:
Arvilommi, H
机构:
[1] National Public Health Institute, Department in Turku, Turku
[2] National Public Health Institute, Department in Turku, Turku
来源:
关键词:
ELISPOT;
enzyme-linked immunospot assay;
antibody-secreting cells;
infection;
vaccine immunogenicity;
PERIPHERAL-BLOOD LYMPHOCYTES;
ORAL CHOLERA VACCINATION;
INTESTINAL IMMUNE-RESPONSES;
SHIGELLA-SONNEI VACCINE;
B CONJUGATE VACCINE;
SALMONELLA-TYPHI;
INTERFERON-GAMMA;
CYTOKINE PRODUCTION;
IMMUNOSPOT ASSAY;
CLONAL ANALYSIS;
D O I:
10.1111/j.1699-0463.1996.tb00734.x
中图分类号:
R392 [医学免疫学];
Q939.91 [免疫学];
学科分类号:
100102 ;
摘要:
Based on immunoassay principles, methods have been developed for the analysis of secreted products at the cellular level. This approach offers substantial methodological advantages compared to traditional immunoassays. In a number of applications cell-based methods have proved able to overcome many of the problems inherent to immunoassays of biological fluids. This review focuses on applications of ELISPOT in natural infections and vaccinations of human individuals. The studies reviewed here have contributed to our understanding of the B-cell responses in infections and the independence of mucosal and systemic immune responses. Whilst diagnostic applications are rare, enzyme immunospot assays have been extensively used in testing the immunogenicity of vaccines. In particular, B-cell responses to mucosal vaccines are better covered with this cellular assay.
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页码:401 / 410
页数:10
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