ELISPOT for detecting antibody-secreting cells in response to infections and vaccination

被引:15
作者
Arvilommi, H
机构
[1] National Public Health Institute, Department in Turku, Turku
[2] National Public Health Institute, Department in Turku, Turku
关键词
ELISPOT; enzyme-linked immunospot assay; antibody-secreting cells; infection; vaccine immunogenicity; PERIPHERAL-BLOOD LYMPHOCYTES; ORAL CHOLERA VACCINATION; INTESTINAL IMMUNE-RESPONSES; SHIGELLA-SONNEI VACCINE; B CONJUGATE VACCINE; SALMONELLA-TYPHI; INTERFERON-GAMMA; CYTOKINE PRODUCTION; IMMUNOSPOT ASSAY; CLONAL ANALYSIS;
D O I
10.1111/j.1699-0463.1996.tb00734.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Based on immunoassay principles, methods have been developed for the analysis of secreted products at the cellular level. This approach offers substantial methodological advantages compared to traditional immunoassays. In a number of applications cell-based methods have proved able to overcome many of the problems inherent to immunoassays of biological fluids. This review focuses on applications of ELISPOT in natural infections and vaccinations of human individuals. The studies reviewed here have contributed to our understanding of the B-cell responses in infections and the independence of mucosal and systemic immune responses. Whilst diagnostic applications are rare, enzyme immunospot assays have been extensively used in testing the immunogenicity of vaccines. In particular, B-cell responses to mucosal vaccines are better covered with this cellular assay.
引用
收藏
页码:401 / 410
页数:10
相关论文
共 70 条
  • [1] ANTIBODY-SYNTHESIS IN ROACH (RUTILUS-RUTILUS) - ANALYSIS OF ANTIBODY-SECRETING CELLS IN LYMPHOID ORGANS WITH ELISPOT-ASSAY
    AALTONEN, TM
    JOKINEN, EI
    VALTONEN, ET
    [J]. FISH & SHELLFISH IMMUNOLOGY, 1994, 4 (02) : 129 - 140
  • [2] INFLUENCE OF PREVACCINATION IMMUNITY ON THE HUMAN LYMPHOCYTE-B RESPONSE TO A HAEMOPHILUS-INFLUENZAE TYPE-B CONJUGATE VACCINE
    BARINGTON, T
    KRISTENSEN, K
    HENRICHSEN, J
    HEILMANN, C
    [J]. INFECTION AND IMMUNITY, 1991, 59 (03) : 1057 - 1064
  • [3] QUANTITATION OF ANTIBODY-SECRETING CELLS IN THE BLOOD AFTER VACCINATION WITH A HAEMOPHILUS-INFLUENZAE TYPE-B CONJUGATE VACCINE
    BARINGTON, T
    HEILMANN, C
    ANDERSEN, V
    [J]. SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1990, 31 (04) : 515 - 522
  • [4] HEAVY-CHAIN ISOTYPE PATTERNS OF HUMAN ANTIBODY-SECRETING CELLS INDUCED BY HAEMOPHILUS-INFLUENZAE TYPE-B CONJUGATE VACCINES IN RELATION TO AGE AND PREIMMUNITY
    BARINGTON, T
    JUUL, L
    GYHRS, A
    HEILMANN, C
    [J]. INFECTION AND IMMUNITY, 1994, 62 (08) : 3066 - 3074
  • [5] THE USE OF MONOCLONAL-ANTIBODIES IN AN ENZYME IMMUNOSPOT ASSAY TO DETECT ISOTYPE-SPECIFIC ANTIBODY-SECRETING CELLS IN PIGS AND CHICKENS
    BIANCHI, ATJ
    SCHOLTEN, JW
    JONGENELEN, IMCA
    KOCH, G
    [J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1990, 24 (02) : 125 - 134
  • [6] ANTIBODY CLASS AND SUBCLASS RESPONSES TO PNEUMOCOCCAL POLYSACCHARIDES FOLLOWING IMMUNIZATION OF HUMAN IMMUNODEFICIENCY VIRUS-INFECTED PATIENTS
    CARSON, PJ
    SCHUT, RL
    SIMPSON, ML
    OBRIEN, J
    JANOFF, EN
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1995, 172 (02) : 340 - 345
  • [7] AN EARLY HUMORAL IMMUNE-RESPONSE IN PERIPHERAL-BLOOD FOLLOWING PARENTERAL INACTIVATED INFLUENZA VACCINATION
    COX, RJ
    BROKSTAD, KA
    ZUCKERMAN, MA
    WOOD, JM
    HAAHEIM, LR
    OXFORD, JS
    [J]. VACCINE, 1994, 12 (11) : 993 - 999
  • [8] ACTIVATION OF HUMAN PERIPHERAL-BLOOD B-CELLS FOLLOWING IMMUNIZATION WITH HEPATITIS-B SURFACE-ANTIGEN VACCINE
    CUPPS, TR
    GOLDSMITH, PK
    VOLKMAN, DJ
    GERIN, JL
    PURCELL, RH
    FAUCI, AS
    [J]. CELLULAR IMMUNOLOGY, 1984, 86 (01) : 145 - 154
  • [9] REVERSE ELISPOT ASSAY FOR CLONAL ANALYSIS OF CYTOKINE PRODUCTION .1. ENUMERATION OF GAMMA-INTERFERON-SECRETING CELLS
    CZERKINSKY, C
    ANDERSSON, G
    EKRE, HP
    NILSSON, LA
    KLARESKOG, L
    OUCHTERLONY, O
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1988, 110 (01) : 29 - 36
  • [10] ANTIBODY-PRODUCING CELLS IN PERIPHERAL-BLOOD AND SALIVARY-GLANDS AFTER ORAL CHOLERA VACCINATION OF HUMANS
    CZERKINSKY, C
    SVENNERHOLM, AM
    QUIDING, M
    JONSSON, R
    HOLMGREN, J
    [J]. INFECTION AND IMMUNITY, 1991, 59 (03) : 996 - 1001