Detection and characterization of phospholipase D by flow injection analysis

被引：9

作者：

Becker, M

Spohn, U

UlbrichHofmann, R

机构：

[1] Martin-Luther-Univ. Halle/Wittenberg, Institut für Biotechnologie, D-06120 Halle

来源：

ANALYTICAL BIOCHEMISTRY | 1997年 / 244卷 / 01期

关键词：

D O I：

10.1006/abio.1996.9864

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A precisely working automated system for the investigation of phospholipases D (PLDs, EC 3.1.4.4) from plant and microbial sources with how injection analysis (FIA) has been developed. The two versions of the FIA setup described are based on the oxidation of choline liberated from phosphatidylcholine by PLD action and catalyzed by choline oxidase and the chemiluminescence detection of hydrogen peroxide produced by this reaction. The correlation between this chemiluminescence signal and the PLD activity was linear in the range between 1 and 100 mU/ml PLD. The sampling frequency was 12 samples per hour. This method was used to compare three different PLDs from cabbage and microbial sources with respect to their pH optima, temperature stability, effecters, and v/[S] characteristics. (C) 1997 Academic Press, Inc.

引用

页码：55 / 61

页数：7

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