Characterisation of human dental stem cells and buccal mucosa fibroblasts

被引：159

作者：

Lindroos, Bettina ^{[1
,2
]}

Maeenpaeae, Katja ^{[1
,2
,3
]}

Ylikomi, Timo ^{[3
,4
]}

Oja, Hannu ^{[5
]}

Suuronen, Riitta ^{[1
,2
,6
,7
]}

Miettinen, Susanna ^{[1
,2
]}

机构：

[1] Univ Tampere, REGEA, Inst Regenerat Med, Tampere 33520, Finland

[2] Tampere Univ Hosp, Tampere 33520, Finland

[3] Univ Tampere, Sch Med, Cell Res Ctr, FIN-33101 Tampere, Finland

[4] Univ Hosp, Dept Clin Chem, Tampere, Finland

[5] Univ Tampere, Tampere Sch Publ Hlth, FIN-33101 Tampere, Finland

[6] Tampere Univ Hosp, Dept Eye Ear & Oral Dis, Tampere, Finland

[7] Tampere Univ Technol, Dept Biomed Engn, FIN-33101 Tampere, Finland

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 368卷 / 02期

关键词：

FACS; dental pulp; dental follicle; periodontal ligament; buccal mucosa fibroblast; osteogenesis; adipogenesis; chondrogenesis;

D O I：

10.1016/j.bbrc.2008.01.081

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human craniofacial stem cells are recently discovered sources of putative mesenchymal stem cells that hold great promise for autogenic or allogenic cell therapy and tissue engineering. Prior to employing these cells in clinical applications, they must be thoroughly investigated and characterized. In this study, the surface marker expression was investigated on dental pulp stem cells (DPSCs), dental follicle cells (DFCs), periodontal ligament stem cells (PDLSCs), and buccal mucosa fibroblasts (BMFs) utilising surface markers for flow cytometry. The osteogenic potential was also examined by bone-associated markers alkaline phosphatase, Runx2, collagen type 1, osteocalcin, and osteopontin. The results from our study demonstrate that the dental cell sources exhibit comparable surface marker and bone-associated marker profiles parallel to those of other mesenchymal stem cell sources, yet distinct from the buccal mucosa fibroblasts. Our data support evidence towards clinical applicability of dental stem cells in hard tissue regeneration. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：329 / 335

页数：7

共 22 条

[21] Human adipose tissue is a source of multipotent stem cells [J].

Zuk, PA ;

Zhu, M ;

Ashjian, P ;

De Ugarte, DA ;

Huang, JI ;

Mizuno, H ;

Alfonso, ZC ;

Fraser, JK ;

Benhaim, P ;

Hedrick, MH .

MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (12) :4279-4295

[22] Multilineage cells from human adipose tissue: Implications for cell-based therapies [J].

Zuk, PA ;

Zhu, M ;

Mizuno, H ;

Huang, J ;

Futrell, JW ;

Katz, AJ ;

Benhaim, P ;

Lorenz, HP ;

Hedrick, MH .

TISSUE ENGINEERING, 2001, 7 (02) :211-228

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