An efficient cell-free protein synthesis system using periplasmic phosphatase-removed S30 extract

被引:12
作者
Kang, SH
Oh, TJ
Kim, RG
Kang, TJ
Hwang, SH
Lee, EY
Choi, CY [1 ]
机构
[1] Seoul Natl Univ, Coll Engn, Sch Chem Engn, Seoul 151742, South Korea
[2] Seoul Natl Univ, Coll Engn, Interdisciplinary Program Biochem Engn & Biotechn, Seoul 151742, South Korea
[3] Kyungsung Univ, Coll Engn, Dept Food Sci & Technol, Pusan 608736, South Korea
关键词
cell-free translation; phosphatase; spheroplast;
D O I
10.1016/S0167-7012(00)00206-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An efficient cell-free translation system was developed by removal of phosphatase localized in the periplasmic space, which hampers the translation reaction by hydrolyzing ATP. S30 extract was prepared from the spheroplast of Escherichia coli, and as much as 40% of ATP-hydrolysis activity of phosphatases could be easily removed by the spheroplast formation. The reaction period of translation using phosphatase-removed S30 extract could be prolonged and protein synthesis was enhanced by more than 30%. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:91 / 96
页数:6
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