Development of primary cell cultures from the penaeid shrimps Penaeus vannamei and P-indicus

被引：35

作者：

Toullec, JY ^{[1
]}

Crozat, Y ^{[1
]}

Patrois, J ^{[1
]}

Porcheron, P ^{[1
]}

机构：

[1] IFREMER, CTR OCEANOL PACIF, TARAVAO, FRANCE

来源：

JOURNAL OF CRUSTACEAN BIOLOGY | 1996年 / 16卷 / 04期

关键词：

D O I：

10.2307/1549183

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

Tissues and dissociated cells from various organs of the shrimp Penaeus vannamei, as well as embryos of P. indicus, were cultured in different media. Cell outgrowths were observed with ovaries, epidermal regeneration buds, and embryos. Optimal results, i.e., a survival for several months, were obtained in M199-based medium for ovaries and embryos, and in Grace's medium for regeneration buds. In every case, a 10% fetal bovine serum supplement was needed.

引用

页码：643 / 649

页数：7

共 26 条

[11]

DRACH P, 1967, VIE MILIEU A BIOL MA, V18, P595

[12] OBSERVATIONS ON THE PRIMARY CULTURE OF HEMOCYTES OF PENAEUS [J].