Laboratory temperature variation is a previously unrecognized source of genotyping error during capillary electrophoresis

被引:34
作者
Davison, A [1 ]
Chiba, S [1 ]
机构
[1] Tohoku Univ, Grad Sch Life Sci, Div Ecol & Evolutionary Biol, Aoba Ku, Sendai, Miyagi 9808578, Japan
来源
MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 02期
关键词
ABI; 310; capillary electrophoresis; genotyping; land snail; microsatellite; temperature;
D O I
10.1046/j.1471-8286.2003.00418.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Capillary electrophoresis is being used increasingly for microsatellite genotyping. Here, we report that laboratory temperature greatly affects the estimated allele size, with 5 degreesC changing the estimated allele size by up to 0.7 bp. With accurate room temperature control, this variation would be relatively unimportant but unfortunately, a questionnaire shows that daily and seasonal laboratory temperature differences are often substantial. One solution is to use a set of reference genotypes, which also greatly assists comparisons between different platforms.
引用
收藏
页码:321 / 323
页数:3
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