EGCG, a major component of green tea, inhibits tumour growth by inhibiting VEGF induction in human colon carcinoma cells

被引:259
作者
Jung, YD
Kim, MS
Shin, BA
Chay, KO
Ahn, BW
Liu, W
Bucana, CD
Gallick, GE
Ellis, LM
机构
[1] Univ Texas, MD Anderson Canc Ctr, Dept Canc Biol, Houston, TX 77030 USA
[2] Univ Texas, MD Anderson Canc Ctr, Dept Surg Oncol, Houston, TX 77030 USA
[3] Chonnam Natl Univ, Sch Med, Res Inst Med Sci, Kwangju 501190, South Korea
关键词
epigallocatechin gallate (EGCG); vascular endothelial growth factor (VEGF); colon carcinoma; Erk-1; Erk-2; angiogenesis;
D O I
10.1054/bjoc.2000.1691
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Catechins are key components of teas that have antiproliferative properties. We investigated the effects of green tea catechins on intracellular signalling and VEGF induction in vitro in serum-deprived HT29 human colon cancer cells and in vivo on the growth of HT29 cells in nude mice. In the in vitro studies, (-)-epigallocatechin gallate (EGCG), the most abundant catechin in green tea extract, inhibited Erk-1 and Erk-2 activation in a dose-dependent manner. However, other tea catechins such as (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), and (-)-epicatechin (EC) did not affect Erk-1 or 2 activation at a concentration of 30 muM. EGCG also inhibited the increase of VEGF expression and promoter activity induced by serum starvation. In the in vivo studies, athymic BALB/c nude mice were inoculated subcutaneously with HT29 cells and treated with daily intraperitoneal injections of EC (negative control) or EGCG at 1.5 mg day(-1) mouse(-1) starting 2 days after tumour cell inoculation. Treatment with EGCG inhibited tumour growth (58%), microvessel density (30%), and tumour cell proliferation (27%) and increased tumour cell apoptosis (1.9-fold) and endothelial cell apoptosis (3-fold) relative to the control condition (P< 0.05 for all comparisons). EGCG may exert at least part of its anticancer effect by inhibiting angiogenesis through blocking the induction of VEGF. (C) 2001 Cancer Research Campaign http://www.bjcancer.com.
引用
收藏
页码:844 / 850
页数:7
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