Induction of phosphorylated-Stat3 following focal cerebral ischemia in mice

被引:59
作者
Wen, TC [1 ]
Peng, H [1 ]
Hata, R [1 ]
Desaki, J [1 ]
Sakanaka, M [1 ]
机构
[1] Ehime Univ, Sch Med, Dept Anat, Shigenobu, Ehime 7910295, Japan
关键词
stat3; transcription factor; middle cerebral artery occlusion; brain; DNA fragmentation; mice;
D O I
10.1016/S0304-3940(01)01711-6
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
It has been shown that Stat3 is induced following transient cerebral ischemia in rat. However there is no evidence that cerebral ischemia stimulates the expression of phosphorylated-Stat3 (p-Stat3), which can activate cytokine-mediated signal transduction from the membrane to the nucleus. In the present study, we investigated the changes in p-Stat3 expression following middle cerebral artery occlusion in mice. Western blot analysis revealed a significant increase in the p-Stat3 protein in the peripheral part of the ischemic area, starting from 6 h after ischemia. p-Stat3 immuno reactivity was detected only in neurons, but not in astrocytes or microglia, and p-Stat3-positive neurons were increased in number in the peripheral part of the ischemic area at 24 h after ischemia. Double staining with aTdT-mediated biotinylated UTP nick end labeling (TUNEL) kit and the p-Stat3 antibody indicated that p-Stat3-positive neurons were also TU NEL-positive. Subsequent immune-electron microscopic observations showed that p-Stat3-positive neurons were at different stages of degeneration. The present findings suggest that the increased expression of p-Stat3 after cerebral ischemia could play a crucial role in ischemia-induced neuron death. (C) 2001 Published by Elsevier Science Ireland Ltd.
引用
收藏
页码:153 / 156
页数:4
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