Identification of cytochrome P450 and glutathione-S-transferase genes preferentially expressed in chernosensory organs of the swallowtail butterfly, Papilio xuthus L.

被引：32

作者：

Ono, H ^{[1
]}

Ozaki, K ^{[1
]}

Yoshikawa, H ^{[1
]}

机构：

[1] JT Biohist Res Hall, Takatsuki, Osaka 5691126, Japan

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2005年 / 35卷 / 08期

关键词：

Papilio xuthus L; cytochrome P450; glutathione-S-transferase; chemoreception; tarsal chemosensillum;

D O I：

10.1016/j.ibmb.2005.03.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The swallowtail butterfly, Papilio xuthus L., feeds exclusively on members of the plant family, Rutaceae. Female butterflies lay eggs in response to specific chemicals contained in their host plants. They perceive a variety of polar compounds as oviposition stimulants through the tarsal chemosensilla of the foreleg by drumming upon the leaf surface. We undertook an expressed sequence tag (EST) analysis to identify the chemosensory-related genes that are expressed in chemosehsilla on the tarsus of P. xuthus. Several genes that showed similarity with biotransformation enzymes were identified from the ESTs. Among them, a cytochrome P450 and a glutathione-S-transferase (GST) were preferentially expressed in the chemosensory organs. We have determined the structure of both cDNA and genomic sequences encoding these enzymes and designated the P450 as CYP341A2, a novel member of CYP341A subfamily, and the GST as GST-pxcs1, respectively. We observed a localized expression of CYP341A2 at the base of tarsal chemosensilla by in situ hybridization. These results suggest that these degrading enzymes play a role in the chemosensory reception for host plant recognition. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：837 / 846

页数：10

共 34 条

[21] OVIPOSITION BEHAVIOR IN LEPIDOPTERA [J].