A novel method of prediction and optimization for preparative high-performance liquid chromatography separation

被引：19

作者：

Jin, Yu ^{[1
]}

Xue, Xingya ^{[1
]}

Liu, Yanfang ^{[1
]}

Xiao, Yuansheng ^{[1
]}

Zhang, Jing ^{[1
]}

Shi, Hui ^{[2
]}

Zhang, Feifang ^{[1
]}

Liang, Xinmiao ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Grad Sch, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

[2] E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1183卷 / 1-2期

关键词：

prediction; optimization; preparative high-performance liquid chromatography (preparative HPLC); retention equation; complex sample; traditional Chinese medicine (TCM);

D O I：

10.1016/j.chroma.2008.01.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The preparation of components from a complex sample is a difficult task. The optimization of the separation and subsequent scale-up is usually carried out by trial and error. In this study, the relationship between retention parameters a and c for analytical and preparative separations was developed when the same solid adsorbent and mobile phase were used. The prediction and optimization of the preparative separation of a complex sample could be achieved by direct and simple conversion of the experimentally determined data from an analytical level. The novel method was successfully applied to optimize the separation of target compounds in traditional Chinese medicine (TCM). (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：76 / 86

页数：11

共 25 条

[1]

Concentration dependence of lumped mass transfer coefficients - Linear versus non-linear chromatography and isocratic versus gradient operation [J].