GRADIENT ELUTION IN PREPARATIVE REVERSED-PHASE LIQUID-CHROMATOGRAPHY

Reversed-phase gradient elution is becoming a method of choice for the laboratory-scale preparative separation of complex mixtures and for the industrial-scale purification of biopolymers. Understanding of band broadening under overloaded conditions in gradient elution is also developing and many papers based on the simulation of elution profiles have recently been published. They are reviewed with emphasis on the resulting optimization schemes for gradient elution preparative separations. The specific problems connected with the preparative chromatography of peptides and proteins under gradient elution conditions are not discussed.