PREPARATIVE LIQUID-CHROMATOGRAPHY .2. EXISTENCE OF OPTIMUM INJECTION CONDITIONS FOR OVERLOADED GRADIENT ELUTION SEPARATIONS

被引：5

作者：

CRETIER, G

ELKHABCHI, M

ROCCA, JL

机构：

[1] Laboratoire des Sciences Analytiques (UA CNRS 435), Université Claude Bernard Lyon I, 69622 Villeurbanne Cedex, Bat. 308, 43 Boulevard du

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 596卷 / 01期

关键词：

D O I：

10.1016/0021-9673(92)80198-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The effect of injection conditions on overloaded gradient elution separations in liquid chromatography was studied theoretically from simulated chromatograms. The simulation algorithm was based on the Craig machine. The separation of binary mixtures having both constant and non-constant separation factors with changing modulator concentration was examined. It appears that there is a general parallelism between gradient elution and isocratic elution under overload conditions: the injection concentration has to be optimized in order to maximize the recovered amount and the optimum injection conditions depend on the column efficiency.

引用

页码：15 / 25

页数：11

共 16 条

[1] GRADIENT ELUTION IN NON-LINEAR PREPARATIVE LIQUID-CHROMATOGRAPHY [J].

ANTIA, FD ;

HORVATH, C .

JOURNAL OF CHROMATOGRAPHY, 1989, 484 :1-27

[2] PREPARATIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY UNDER GRADIENT CONDITIONS .1. BAND BROADENING IN GRADIENT ELUTION AS A FUNCTION OF SAMPLE-SIZE [J].

COX, GB ;

SNYDER, LR ;

DOLAN, JW .

JOURNAL OF CHROMATOGRAPHY, 1989, 484 :409-423

[3] PREPARATIVE SEPARATION OF PEPTIDE AND PROTEIN SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH GRADIENT ELUTION .2. EXPERIMENTAL EXAMPLES COMPARED WITH THEORY [J].

COX, GB ;

ANTLE, PE ;

SNYDER, LR .

JOURNAL OF CHROMATOGRAPHY, 1988, 444 :325-344

[4] PREPARATIVE LIQUID-CHROMATOGRAPHY .1. INFLUENCE OF COLUMN EFFICIENCY ON OPTIMUM INJECTION CONDITIONS UNDER ISOCRATIC ELUTION [J].

CRETIER, G ;

MACHEREL, L ;

ROCCA, JL .

JOURNAL OF CHROMATOGRAPHY, 1992, 590 (02) :175-188

[5]

CRETIER G, 1987, SEPAR SCI TECHNOL, V23, P1881

[6] SIMPLIFIED DESCRIPTION OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION UNDER OVERLOAD CONDITIONS, BASED ON THE CRAIG DISTRIBUTION MODEL .5. GRADIENT ELUTION SEPARATION [J].

EBLE, JE ;

GROB, RL ;

ANTLE, PE ;

SNYDER, LR .

JOURNAL OF CHROMATOGRAPHY, 1987, 405 :51-66

[7] EFFECT OF A DIFFERENCE OF THE COLUMN SATURATION CAPACITIES FOR THE 2 COMPONENTS OF A MIXTURE ON THE RELATIVE INTENSITIES OF THE DISPLACEMENT AND TAG-ALONG EFFECTS IN NONLINEAR CHROMATOGRAPHY [J].

GOLSHANSHIRAZI, S ;

GUIOCHON, G .

ANALYTICAL CHEMISTRY, 1990, 62 (02) :217-220

[8]

KARGER BL, 1973, INTRO SEPARATION SCI, P110

[9] OPTIMIZATION OF SAMPLE-SIZE AND SAMPLE VOLUME IN PREPARATIVE LIQUID-CHROMATOGRAPHY [J].

KATTI, A ;

GUIOCHON, G .

ANALYTICAL CHEMISTRY, 1989, 61 (09) :982-990

[10]

Snyder L. R., 1980, HIGH PERFORMANCE LIQ, V1, P208

← 1 2 →