Induction of IGFBP-1 expression by amino acid deprivation of HepG2 human hepatoma cells involves both a transcriptional activation and an mRNA stabilization due to its 3′UTR

被引：29

作者：

Averous, J ^{[1
]}

Maurin, AC ^{[1
]}

Bruhat, A ^{[1
]}

Jousse, C ^{[1
]}

Arliguie, C ^{[1
]}

Fafournoux, P ^{[1
]}

机构：

[1] INRA, Unite Nutr & Metab Prot, F-63122 St Genes Champanelle, France

来源：

FEBS LETTERS | 2005年 / 579卷 / 12期

关键词：

IGFBP-1; amino acid; transcription; mRNA stability; gene expression;

D O I：

10.1016/j.febslet.2005.03.077

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A dramatic overexpression of IGFBP-I is responsible for growth inhibition, in response to a low-protein diet feeding. It has been demonstrated that a fall in the amino acid concentration was directly responsible for IGFBP-1 induction. In this report, we sought to determine the mechanism by which amino acid limitation upregulates IGFBP-1 expression. Our results show that both transcriptional activation and mRNA stabilization are involved. We also demonstrate that (i) the mGCN2/ATF4 pathway is not involved in this regulation and (ii) the 3'UTR of IGFBP-I mRNA is responsible for its destabilization and regulates its stability in response to amino acid starvation. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2609 / 2614

页数：6