In situ identification of polyphosphate- and polyhydroxyalkanoate-accumulating traits for microbial populations in a biological phosphorus removal process

被引:176
作者
Liu, WT [1 ]
Nielsen, AT
Wu, JH
Tsai, CS
Matsuo, Y
Molin, S
机构
[1] Natl Cent Univ, Grad Inst Environm Engn, Chungli 32054, Taiwan
[2] Tech Univ Denmark, Dept Microbiol, Mol Microbial Ecol Grp, DK-2800 Lyngby, Denmark
[3] Natl Cheng Kung Univ, Dept Environm Engn, Tainan 70101, Taiwan
[4] Chuo Univ, Dept Civil Engn, Tokyo 112, Japan
关键词
D O I
10.1046/j.1462-2920.2001.00164.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Polyphosphate- and polyhydroxyalkanoate (PHA)- accumulating traits of predominant microorganisms in an efficient enhanced biological phosphorus removal (EBPR) process were investigated systematically using a suite of non-culture-dependent methods. Results of 16S rDNA clone library and fluorescence in situ hybridization (FISH) with rRNA-targeted, group-specific oligonucleotide probes indicated that the microbial community consisted mostly of the alpha- (9.5% of total cells), beta- (41.3%) and gamma- (6.8%) subclasses of the class Proteobacteria, Flexibacter-Cytophaga (4.5%) and the Gram-positive high G+C (HGC) group (17.9%), With individual phylogenetic groups or subgroups, members of Candidatus Accumulibacter phosphatis in the beta -2 subclass, a novel HGC group closely related to Tetrasphaera spp., and a novel gamma -proteobacterial group were the predominant populations. Furthermore, electron microscopy with energy-dispersive X-ray analysis was used to validate the staining specificity of 4,6-diamino-2-phenylindole (DAPI) for intracellular polyphosphate and revealed the composition of polyphosphate granules accumulated in predominant bacteria as mostly P, Ca and Na. As a result, DAPI and PHA staining procedures could be combined with FISH to identify directly the polyphosphate- and PHA-accumulating traits of different phylogenetic groups. Members of Accumulibacter phosphatis and the novel gamma-proteobacterial group were observed to accumulate both polyphosphate and PHA. In addition, one novel rod-shaped group, closely related to coccus-shaped Tetrasphaera, and one filamentous group resembling Candidatus Nostocoidia limicola in the HGC group were found to accumulate polyphosphate but not PHA, No cellular inclusions were detected in most members of the alpha -Proteobacteria and the Cytophaga-Flavobacterium group. The diversified functional traits observed suggested that different substrate metabolisms were used by predominant phylogenetic groups in EBPR processes.
引用
收藏
页码:110 / 122
页数:13
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