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I-phenylethynylpyrene (I-PEPy) as refined Excimer forming alternative to pyrene: Case of DNA major groove Excimer

被引：40
作者：
Astakhova, Irina V.
Malakhov, Andrei D.
Stepanova, Irina A.
Ustinov, Alexey V.
Bondarev, Stanislav L.
Paramonov, Alexander S.
Korshun, Vladimir A.
机构：
[1] Shemyakin Ovichinnikov Inst Bioorgan Chem, Moscow 117997, Russia
[2] Inst Mol & Atom Phys, Minsk 220072, BELARUS
来源：
BIOCONJUGATE CHEMISTRY | 2007年 / 18卷 / 06期
关键词：
D O I：
10.1021/bc700280h
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
1-Phenylethynylpyrene fluorochrome was studied as meta- and para-derivatives of arabino-uridine-2'-carbamates in ss and dsDNA. 1-PEPy showed red-shifted emission and increased fluorescence quantum yield compared to pyrene. Although 1-PEPy has very short excited lifetime (< 2.5 ns), it is able to form inter- and intrastrand excimers on DNA, probably resulting from spatial preorganization of two dye molecules.
引用
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页码：1972 / 1980
页数：9
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