I-phenylethynylpyrene (I-PEPy) as refined Excimer forming alternative to pyrene: Case of DNA major groove Excimer

被引：40

作者：

Astakhova, Irina V.

Malakhov, Andrei D.

Stepanova, Irina A.

Ustinov, Alexey V.

Bondarev, Stanislav L.

Paramonov, Alexander S.

Korshun, Vladimir A.

机构：

[1] Shemyakin Ovichinnikov Inst Bioorgan Chem, Moscow 117997, Russia

[2] Inst Mol & Atom Phys, Minsk 220072, BELARUS

来源：

BIOCONJUGATE CHEMISTRY | 2007年 / 18卷 / 06期

关键词：

D O I：

10.1021/bc700280h

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

1-Phenylethynylpyrene fluorochrome was studied as meta- and para-derivatives of arabino-uridine-2'-carbamates in ss and dsDNA. 1-PEPy showed red-shifted emission and increased fluorescence quantum yield compared to pyrene. Although 1-PEPy has very short excited lifetime (< 2.5 ns), it is able to form inter- and intrastrand excimers on DNA, probably resulting from spatial preorganization of two dye molecules.

引用

页码：1972 / 1980

页数：9

共 61 条

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