Measurement of DNA breaks and oxidative damage in polymorphonuclear and mononuclear white blood cells: a novel approach using the comet assay

被引：46

作者：

Giovannelli, L ^{[1
]}

Pitozzi, V ^{[1
]}

Riolo, S ^{[1
]}

Dolara, P ^{[1
]}

机构：

[1] Univ Florence, Dipartimento Farmacol Preclin & Clin, I-50139 Florence, Italy

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2003年 / 538卷 / 1-2期

关键词：

DNA damage; DNA oxidation; granulocytes; lymphocytes;

D O I：

10.1016/S1383-5718(03)00090-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

DNA damage is thought to play a relevant role in degenerative diseases and aging. Therefore, measuring DNA damage in living cells without artifacts is a critical issue, especially with very sensitive methods, such as the comet assay, which can detect very low levels of DNA damage. We show here that the procedures of cell subtype isolation increase DNA damage measured in human white blood cells (WBC) with the comet assay. We describe a novel and simple method to measure DNA strand breaks and oxidative damage separately in polymorphonuclear and mononuclear leukocytes, using whole blood without previous cell isolation. This method can be useful for measuring DNA damage in different subtypes of human peripheral leukocytes, avoiding the artifacts and the time involved in the cell separation procedures. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：71 / 80

页数：10