MicroRNA-26b relieves inflammatory response and myocardial remodeling of mice with myocardial infarction by suppression of MAPK pathway through binding to PTGS2

被引：65

作者：

Ge, Zhen-Wei ^{[1
]}

Zhu, Xi-Liang ^{[1
]}

Wang, Bao-Cai ^{[1
]}

Hu, Jun-Long ^{[1
]}

Sun, Jun-Jie ^{[1
]}

Wang, Sheng ^{[1
]}

Chen, Xian-Jie ^{[1
]}

Meng, Shu-Ping ^{[2
]}

Liu, Lin ^{[3
]}

Cheng, Zhao-Yun ^{[1
]}

机构：

[1] Henan Prov Peoples Hosp, Dept Cardiovasc Surg, 7 Weiwu Rd, Zhengzhou 450003, Henan, Peoples R China

[2] Henan Prov Peoples Hosp, ICU Cardiovasc Surg, Zhengzhou 450003, Henan, Peoples R China

[3] Henan Prov Peoples Hosp, Dept Cardiovasc Ultrasound, Zhengzhou 450003, Henan, Peoples R China

来源：

INTERNATIONAL JOURNAL OF CARDIOLOGY | 2019年 / 280卷

关键词：

MicroRNA-26b; PTGS2; MAPK pathway; Myocardial infarction; Inflammatory response; Myocardial remodeling; CORONARY-ARTERY LIGATION; P38; MAPK; IL-10; EXPRESSION; EFFICIENT MODEL; INHIBITION; APOPTOSIS; PROLIFERATION; MACROPHAGES; ACTIVATION; CARCINOMA;

D O I：

10.1016/j.ijcard.2018.12.077

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Background: Myocardial infarction (MI) is a common cardiovascular disease caused by myocardial ischemia. Also, microRNA (miRNA) participates in the pathophysiology of many cardiovascular diseases, which can affect stem cell transplantation in the treatment of MI. In this study, our aim is to explore effect of miR-26b on inflammatory response and myocardial remodeling through the MAPK pathway by targeting PTGS2 in mice with MI. Methods: Microarray data analysis was conducted to screen MI-related differentially expressed gens (DEGs). Relationship between miR-26b and PTGS2 was testified. Cardiac function, inflammatory reaction, infarct size, and myocardial fibrosis were observed. The miR-26b expression and mRNA and protein levels of, PTGS2, ERK, JNK and p38 and Bcl-2/Bax were examined. The effect of miR-26b on cell apoptosis was also analyzed. Results: MiR-26b was predicted to target PTGS2 further to mediate the MAPK pathway, thus affecting MI. MiR26b negatively targeted PTGS2. MI mice showed decreased cardiac function, as well as increased inflammatory reaction, myocardial injury, area of fibrosis and myocardial cell apoptosis. After injection of miR-26b agomir or NS-398 (PTGS2 inhibitor), inflammatory response of MI mice was attenuated and myocardial remodeling induced by MI was alleviated. Conclusion: These findings indicate that miR-26b inhibits PTGS2 to activate the MAPK pathway, so as to reduce inflammatory response and improve myocardial remodeling in mice with MI. (c) 2018 Published by Elsevier B.V.

引用

页码：152 / 159

页数：8

共 45 条

[1]

Bromelain inhibits COX-2 expression by blocking the activation of MAPK regulated NF-kappa B against skin tumor-initiation triggering mitochondrial death pathway [J].

Bhui, Kulpreet ;

Prasad, Sahdeo ;

George, Jasmine ;

Shukla, Yogeshwer .

CANCER LETTERS, 2009, 282 (02) :167-176

[2]

MiR-26 down-regulates TNF-α/NF-κB signalling and IL-6 expression by silencing HMGA1 and MALT1 [J].

Chen, Chyi-Ying A. ;

Chang, Jeffrey T. ;

Ho, Yi-Fang ;

Shyu, Ann-Bin .

NUCLEIC ACIDS RESEARCH, 2016, 44 (08) :3772-3787

[3]

Gao E, 2013, METHODS MOL BIOL, V1037, P299, DOI 10.1007/978-1-62703-505-7_17

[4]

A Novel and Efficient Model of Coronary Artery Ligation and Myocardial Infarction in the Mouse [J].

Gao, Erhe ;

Lei, Yong Hong ;

Shang, Xiying ;

Huang, Z. Maggie ;

Zuo, Lin ;

Boucher, Matthieu ;

Fan, Qian ;

Chuprun, J. Kurt ;

Ma, Xin L. ;

Koch, Walter J. .

CIRCULATION RESEARCH, 2010, 107 (12) :1445-+

[5]

PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle [J].

Gao, Long ;

Liu, Bo ;

Mao, Wei ;

Gao, Ruifeng ;

Zhang, Shuangyi ;

Duritahala ;

Fu, Changqi ;

Shen, Yuan ;

Zhang, Ying ;

Zhang, Nan ;

Wu, Jindi ;

Deng, Yang ;

Wu, Xing ;

Cao, Jinshan .

ANIMAL REPRODUCTION SCIENCE, 2017, 187 :54-63

[6]

affy -: analysis of Affymetrix GeneChip data at the probe level [J].

Gautier, L ;

Cope, L ;

Bolstad, BM ;

Irizarry, RA .

BIOINFORMATICS, 2004, 20 (03) :307-315

[7]

Trimetazidine suppresses oxidative stress, inhibits MMP-2 and MMP-9 expression, and prevents cardiac rupture in mice with myocardial infarction [J].

Gong, Wei ;

Ma, Youcai ;

Li, Aobo ;

Shi, Han ;

Nie, Shaoping .

CARDIOVASCULAR THERAPEUTICS, 2018, 36 (05)

[8]

Evaluation of PTGS2 Expression, PIK3CA Mutation, Aspirin Use and Colon Cancer Survival in a Population-Based Cohort Study [J].

Gray, Ronan T. ;

Cantwell, Marie M. ;

Coleman, Helen G. ;

Loughrey, Maurice B. ;

Bankhead, Peter ;

McQuaid, Stephen ;

O'Neill, Roisin F. ;

Arthur, Kenneth ;

Bingham, Victoria ;

McGready, Claire ;

Gavin, Anna T. ;

Cardwell, Chris R. ;

Johnston, Brian T. ;

James, Jacqueline A. ;

Hamilton, Peter W. ;

Salto-Tellez, Manuel ;

Murray, Liam J. .

CLINICAL AND TRANSLATIONAL GASTROENTEROLOGY, 2017, 8

[9]

Cell type-specific regulation of IL-10 expression in inflammation and disease [J].

Hedrich, Christian M. ;

Bream, Jay H. .

IMMUNOLOGIC RESEARCH, 2010, 47 (1-3) :185-206

[10]

Atf3 negatively regulates Ptgs2/Cox2 expression during acute inflammation [J].

Hellmann, Jason ;

Tang, Yunan ;

Zhang, Michael J. ;

Hai, Tsonwin ;

Bhatnagar, Aruni ;

Srivastava, Sanjay ;

Spite, Matthew .

PROSTAGLANDINS & OTHER LIPID MEDIATORS, 2015, 116 :49-56

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