CL22 - a novel cationic peptide for efficient transfection of mammalian cells

被引：34

作者：

Haines, AMR ^{[1
]}

Irvine, AS ^{[1
]}

Mountain, A ^{[1
]}

Charlesworth, J ^{[1
]}

Farrow, NA ^{[1
]}

Husain, RD ^{[1
]}

Hyde, H ^{[1
]}

Ketteringham, H ^{[1
]}

McDermott, R ^{[1
]}

Mulcahy, AF ^{[1
]}

Mustoe, TL ^{[1
]}

Reid, SCH ^{[1
]}

Rouquette, M ^{[1
]}

Shaw, JC ^{[1
]}

Thatcher, DR ^{[1
]}

Welsh, JH ^{[1
]}

Williams, DE ^{[1
]}

Zauner, W ^{[1
]}

Phillips, RO ^{[1
]}

机构：

[1] Cobra Therapeut Ltd, Keele ST5 5SP, Staffs, England

来源：

GENE THERAPY | 2001年 / 8卷 / 02期

关键词：

gene delivery; non-viral; condensing peptide; transfection;

D O I：

10.1038/sj.gt.3301314

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Condensing peptide-DNA complexes have great potential as nonviral agents for gene delivery. To date, however, such complexes have given transfection activities greatly inferior to adenovirus and somewhat inferior to cationic lipid-DNA complexes, even for cell lines and primary cells in vitro. We report here the identification of a novel condensing peptide, CL22, which forms DNA complexes that efficiently transfect many cell lines, as well as primary dendritic and endothelial cells. We report studies with sequence and structure variants that define some properties of the peptide that contribute to efficient transfection. We demonstrate that the superior transfection activity of CL22 compared with other DNA condensing peptides is conferred at a step after uptake of the complexes into cells. We show that CL22-DNA complexes have transfection activity that is at least equivalent to the best available nonviral agents.

引用

页码：99 / 110

页数：12

共 36 条

[11] Gene transfer mediated by polyarginine requires a formation of big carrier-complex of DNA aggregate [J].